TY - JOUR

T1 - Comparison of DNA complexation with antitumor agent cis-DDP and binuclear bivalent platinum compound containing pyrazine

AU - Kas'yanenko, N. A.

AU - Aia, E. E.F.

AU - Bogdanov, A. A.

AU - Kosmotynskaya, Yu V.

AU - Yakovlev, K. I.

N1 - Funding Information: ACKNOWLEDGMENTS The authors thank E.B. Moroshkina for useful advice. This work was supported in part by the Russian Foundation for Basic Research (project no. 00-04-49014). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - Conformational properties of DNA molecule upon its complexation with binuclear compounds of bivalent platinum in the cis configuration containing pyrazine ligand were studied by circular dichroism, viscometry, and dynamic birefringence. Comparison with an active antitumor agent cis-diamminedichloroplatinum (cis-DDP) was made. Experimental data indicate that interaction of these compounds with DNA results in the formation of a coordination bond of platinum with nucleic bases. The structure of the complex depends on the ratio of platinum and DNA concentrations in the initial solution. The study of DNA protonation in complex with the binuclear coordination compound showed that the binding of platinum with DNA bases involves the N7 atom of guanine. Competition was observed between the studied compound and cis-DDP for the binding site on DNA. The macromolecule binds stronger with the binuclear platinum compound than with cis-DDP.

AB - Conformational properties of DNA molecule upon its complexation with binuclear compounds of bivalent platinum in the cis configuration containing pyrazine ligand were studied by circular dichroism, viscometry, and dynamic birefringence. Comparison with an active antitumor agent cis-diamminedichloroplatinum (cis-DDP) was made. Experimental data indicate that interaction of these compounds with DNA results in the formation of a coordination bond of platinum with nucleic bases. The structure of the complex depends on the ratio of platinum and DNA concentrations in the initial solution. The study of DNA protonation in complex with the binuclear coordination compound showed that the binding of platinum with DNA bases involves the N7 atom of guanine. Competition was observed between the studied compound and cis-DDP for the binding site on DNA. The macromolecule binds stronger with the binuclear platinum compound than with cis-DDP.

KW - Binuclear complexes of bivalent platinum

KW - Circular dichroism

KW - cis-DDP

KW - DNA

KW - Hydrodynamic methods

KW - Protonated DNA

UR - http://www.scopus.com/inward/record.url?scp=0141500182&partnerID=8YFLogxK

U2 - 10.1023/A:1019877907974

DO - 10.1023/A:1019877907974

M3 - Article

AN - SCOPUS:0141500182

VL - 36

SP - 594

EP - 600

JO - Molecular Biology

JF - Molecular Biology

SN - 0026-8933

IS - 4

ER -