TY - CONF

T1 - Comparative genomics and quantitative proteomics reveal differentially produced proteins underlying virulence and host specificity in Bacillus thuringiensis.

AU - Malovichko, Yury

AU - Gorbach, Daria

AU - Romanovskaya, Ekaterina

AU - Lukasheva, Elena

AU - Nizhnikov, Anton

AU - Antonets, Kirill

N1 - Conference code: 12

PY - 2020

Y1 - 2020

N2 - Bacillus thuringiensis is a Gram-positive spore- forming bacterium known for its insecticidal activities. Although its features of high virulence and exceptional host specificity are wiely known and have conditioned its use as a source of novel biopesticides, molecular mechanisms underlying these traits remain elusive and are usually attributed to its repertoire of proteinaceous toxins. In this work we used combined proteogenomic approach to dissect <>. We used three different strains of B. thuringiensis belonging to the var. thuringiensis, darmstadtiensis and israeliensis and one derivate of B. thuringiensis var israeliensis, which lost the ability to produce Cry-toxins. By using hybrid Oxford Nanopore and Illumina sequencing we achieved replicon-level genome assemblies of the studied strains which, upon annotation, facilitated comparison of virulence factor repertoires and putative reasons of virulence loss in derivate strain, Further application of quantitative HPLC-Orbitrap-MS and proteome level-com

AB - Bacillus thuringiensis is a Gram-positive spore- forming bacterium known for its insecticidal activities. Although its features of high virulence and exceptional host specificity are wiely known and have conditioned its use as a source of novel biopesticides, molecular mechanisms underlying these traits remain elusive and are usually attributed to its repertoire of proteinaceous toxins. In this work we used combined proteogenomic approach to dissect <>. We used three different strains of B. thuringiensis belonging to the var. thuringiensis, darmstadtiensis and israeliensis and one derivate of B. thuringiensis var israeliensis, which lost the ability to produce Cry-toxins. By using hybrid Oxford Nanopore and Illumina sequencing we achieved replicon-level genome assemblies of the studied strains which, upon annotation, facilitated comparison of virulence factor repertoires and putative reasons of virulence loss in derivate strain, Further application of quantitative HPLC-Orbitrap-MS and proteome level-com

KW - bacillus thuringiensis

KW - biopesticides

KW - genomics

KW - illumina

KW - nanopore

KW - orbitrap

KW - pathogenicity

KW - proteomics

KW - specificity

KW - toxins

KW - virulence

KW - bacillus thuringiensis

KW - biopesticides

KW - genomics

KW - illumina

KW - nanopore

KW - orbitrap

KW - pathogenicity

KW - proteomics

KW - specificity

KW - toxins

KW - virulence

M3 - Abstract

SP - 62

EP - 63

T2 - Bioinformatics of Genome Regulation and Structure/Systems Biology

Y2 - 6 July 2020 through 10 July 2020

ER -