Результаты исследований: Научные публикации в периодических изданиях › Обзорная статья › Рецензирование
Comparative Analyses of Zebrafish Anxiety-Like Behavior Using Conflict-Based Novelty Tests. / Kysil, Elana V.; Meshalkina, Darya A.; Frick, Erin E.; Echevarria, David J.; Rosemberg, Denis B.; Maximino, Caio; Lima, Monica Gomes; Abreu, Murilo S.; Giacomini, Ana C.; Barcellos, Leonardo J.G.; Song, Cai; Kalueff, Allan V.
в: Zebrafish, Том 14, № 3, 06.2017, стр. 197-208.Результаты исследований: Научные публикации в периодических изданиях › Обзорная статья › Рецензирование
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TY - JOUR
T1 - Comparative Analyses of Zebrafish Anxiety-Like Behavior Using Conflict-Based Novelty Tests
AU - Kysil, Elana V.
AU - Meshalkina, Darya A.
AU - Frick, Erin E.
AU - Echevarria, David J.
AU - Rosemberg, Denis B.
AU - Maximino, Caio
AU - Lima, Monica Gomes
AU - Abreu, Murilo S.
AU - Giacomini, Ana C.
AU - Barcellos, Leonardo J.G.
AU - Song, Cai
AU - Kalueff, Allan V.
N1 - Publisher Copyright: © 2017, Mary Ann Liebert, Inc.
PY - 2017/6
Y1 - 2017/6
N2 - Modeling of stress and anxiety in adult zebrafish (Danio rerio) is increasingly utilized in neuroscience research and central nervous system (CNS) drug discovery. Representing the most commonly used zebrafish anxiety models, the novel tank test (NTT) focuses on zebrafish diving in response to potentially threatening stimuli, whereas the light-dark test (LDT) is based on fish scototaxis (innate preference for dark vs. bright areas). Here, we systematically evaluate the utility of these two tests, combining meta-analyses of published literature with comparative in vivo behavioral and whole-body endocrine (cortisol) testing. Overall, the NTT and LDT behaviors demonstrate a generally good cross-test correlation in vivo, whereas meta-analyses of published literature show that both tests have similar sensitivity to zebrafish anxiety-like states. Finally, NTT evokes higher levels of cortisol, likely representing a more stressful procedure than LDT. Collectively, our study reappraises NTT and LDT for studying anxiety-like states in zebrafish, and emphasizes their developing utility for neurobehavioral research. These findings can help optimize drug screening procedures by choosing more appropriate models for testing anxiolytic or anxiogenic drugs.
AB - Modeling of stress and anxiety in adult zebrafish (Danio rerio) is increasingly utilized in neuroscience research and central nervous system (CNS) drug discovery. Representing the most commonly used zebrafish anxiety models, the novel tank test (NTT) focuses on zebrafish diving in response to potentially threatening stimuli, whereas the light-dark test (LDT) is based on fish scototaxis (innate preference for dark vs. bright areas). Here, we systematically evaluate the utility of these two tests, combining meta-analyses of published literature with comparative in vivo behavioral and whole-body endocrine (cortisol) testing. Overall, the NTT and LDT behaviors demonstrate a generally good cross-test correlation in vivo, whereas meta-analyses of published literature show that both tests have similar sensitivity to zebrafish anxiety-like states. Finally, NTT evokes higher levels of cortisol, likely representing a more stressful procedure than LDT. Collectively, our study reappraises NTT and LDT for studying anxiety-like states in zebrafish, and emphasizes their developing utility for neurobehavioral research. These findings can help optimize drug screening procedures by choosing more appropriate models for testing anxiolytic or anxiogenic drugs.
KW - anxiety-like behavior
KW - behavioral phenotyping
KW - the light-dark test
KW - the novel tank test
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85020182570&partnerID=8YFLogxK
U2 - 10.1089/zeb.2016.1415
DO - 10.1089/zeb.2016.1415
M3 - Review article
C2 - 28459655
AN - SCOPUS:85020182570
VL - 14
SP - 197
EP - 208
JO - Zebrafish
JF - Zebrafish
SN - 1545-8547
IS - 3
ER -
ID: 99381419