TY - JOUR

T1 - Coilin-containing nuclear biomolecular condensates in zebra finch Taeniopygia guttata growing oocytes

AU - Галкина, Светлана Анатольевна

AU - Матвеева, Ксения Сергеевна

AU - Такки, Ольга Дмитриевна

AU - Володькина, Валерия Александровна

AU - Кулак, Мария Михайловна

AU - Шалутина, Юлия Андреевна

AU - Гагинская, Елена Романовна

PY - 2025/5/14

Y1 - 2025/5/14

N2 - In most animals, oocyte growth is accompanied by genome activation, an increase in nuclear volume, and the formation of various biomolecular condensates (BioMCs) through multivalent interactions involving intrinsically disordered protein regions (IDRs) and phase separation. In this study, we characterize specific nuclear biomolecular condensates (NBioMCs) detectable by light microscopy in the oocytes of the zebra finch (Taeniopygia guttata, Passeriformes, Aves), a model species in genomics and neurobiology. We identified a nucleolus in oocytes at the early diplotene stage and observed numerous NBioMCs that tested positive for coilin in oocytes at the lampbrush stage, a period of active transcription. The coilin-positive NBioMCs may be freely distributed within the nucleus or associated with chromosome centromeres. They share characteristics with several known nuclear structures, including nucleoli (due to the presence of fibrillarin and nucleolin), Cajal bodies (marked by coilin and scaRNA2), interchromatin granule clusters (containing SRSF2), and centromeric protein bodies (CPBs) described in other avian species (exhibiting centromeric localization when chromosome-associated and containing STAG2 and SMC5). However, their specific function in zebra finch oocytes remains unclear and requires further investigation.

AB - In most animals, oocyte growth is accompanied by genome activation, an increase in nuclear volume, and the formation of various biomolecular condensates (BioMCs) through multivalent interactions involving intrinsically disordered protein regions (IDRs) and phase separation. In this study, we characterize specific nuclear biomolecular condensates (NBioMCs) detectable by light microscopy in the oocytes of the zebra finch (Taeniopygia guttata, Passeriformes, Aves), a model species in genomics and neurobiology. We identified a nucleolus in oocytes at the early diplotene stage and observed numerous NBioMCs that tested positive for coilin in oocytes at the lampbrush stage, a period of active transcription. The coilin-positive NBioMCs may be freely distributed within the nucleus or associated with chromosome centromeres. They share characteristics with several known nuclear structures, including nucleoli (due to the presence of fibrillarin and nucleolin), Cajal bodies (marked by coilin and scaRNA2), interchromatin granule clusters (containing SRSF2), and centromeric protein bodies (CPBs) described in other avian species (exhibiting centromeric localization when chromosome-associated and containing STAG2 and SMC5). However, their specific function in zebra finch oocytes remains unclear and requires further investigation.

KW - Birds

KW - Cajal bodies

KW - Lampbrush chromosomes

KW - Nuclear biomolecular condensates

KW - Oogenesis

UR - https://www.mendeley.com/catalogue/b0303f80-7d31-3bf4-bea8-95ed3b337487/

U2 - 10.1016/j.ydbio.2025.05.012

DO - 10.1016/j.ydbio.2025.05.012

M3 - Article

VL - 524

SP - 144

EP - 151

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

ER -