Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Clathrin-mediated endocytosis cooperates with bulk endocytosis to generate vesicles. / Arpino, Gianvito; Somasundaram, A; Shin, Wonchul; Ge, L; Villareal, S; Chan, C.Y.; Ashery, U; Шупляков, Олег Викторович; Taraska, J.W.; Wu, L.G.
в: iScience, Том 25, № 2, 103809, 01.02.2022.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Clathrin-mediated endocytosis cooperates with bulk endocytosis to generate vesicles
AU - Arpino, Gianvito
AU - Somasundaram, A
AU - Shin, Wonchul
AU - Ge, L
AU - Villareal, S
AU - Chan, C.Y.
AU - Ashery, U
AU - Шупляков, Олег Викторович
AU - Taraska, J.W.
AU - Wu, L.G.
N1 - Publisher Copyright: © 2022
PY - 2022/2/1
Y1 - 2022/2/1
N2 - Clathrin-mediated endocytosis, the most prominent endocytic mode, is thought to be generated primarily from relatively flat patches of the plasma membrane. By employing conventional and platinum replica electron microscopy and super-resolution STED microscopy in neuroendocrine chromaffin cells, we found that large Ω-shaped or dome-shaped plasma membrane invaginations, previously thought of as the precursor of bulk endocytosis, are primary sites for clathrin-coated pit generation after depolarization. Clathrin-coated pits are more densely packed at invaginations rather than flat membranes, suggesting that invaginations are preferred sites for clathrin-coated pit formation, likely because their positive curvature facilitates coated-pit formation. Thus, clathrin-mediated endocytosis closely collaborates with bulk endocytosis to enhance endocytic capacity in active secretory cells. This direct collaboration between two classically independent endocytic pathways is of broad importance given the central role of both clathrin-mediated endocytosis and bulk endocytosis in neurons, endocrine cells, immune cells, and many other cell types throughout the body.
AB - Clathrin-mediated endocytosis, the most prominent endocytic mode, is thought to be generated primarily from relatively flat patches of the plasma membrane. By employing conventional and platinum replica electron microscopy and super-resolution STED microscopy in neuroendocrine chromaffin cells, we found that large Ω-shaped or dome-shaped plasma membrane invaginations, previously thought of as the precursor of bulk endocytosis, are primary sites for clathrin-coated pit generation after depolarization. Clathrin-coated pits are more densely packed at invaginations rather than flat membranes, suggesting that invaginations are preferred sites for clathrin-coated pit formation, likely because their positive curvature facilitates coated-pit formation. Thus, clathrin-mediated endocytosis closely collaborates with bulk endocytosis to enhance endocytic capacity in active secretory cells. This direct collaboration between two classically independent endocytic pathways is of broad importance given the central role of both clathrin-mediated endocytosis and bulk endocytosis in neurons, endocrine cells, immune cells, and many other cell types throughout the body.
KW - Biological sciences
KW - Cell biology
KW - Endocrine regulation
KW - Neuroscience
KW - VISUALIZATION
KW - FUSION
KW - EXOCYTOSIS
KW - MECHANISMS
KW - RELEASE
KW - FORMS
KW - MEMBRANE RETRIEVAL
KW - MODES
KW - CURVATURE
KW - FISSION
UR - http://www.scopus.com/inward/record.url?scp=85124212810&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/ebac0dde-5c11-34bb-bc61-694ca21f8186/
U2 - 10.1016/j.isci.2022.103809
DO - 10.1016/j.isci.2022.103809
M3 - Article
C2 - 35198874
VL - 25
JO - iScience
JF - iScience
SN - 2589-0042
IS - 2
M1 - 103809
ER -
ID: 91970594