The syringomycins (SRs) are cyclic lipodepsipeptides produced by the phytopathogenic bacterium Pseudomonas syringae pv. syringae. Discovered and described during the 1980s and 1990s, the effects of the SRs on plant cells and inhibition of fungal and yeast growth are due to specific interactions with membrane lipids of target cells. Using one‐side addition of SRs to a system consisting of a bilayer lipid membrane and two aqueous compartments, researchers have shown that the SRs form anion selective channels that possess unique and highly reproducible voltage sensitive properties. Moreover, SRs form two types of ion channels, namely “small” and “large,” that differ several‐fold in their conductance. These initial findings generated interest in the SRs to (1) consider development of the SRs as antifungal agents, and (2) use SR channels as mechanistic models for native ion channels. Follow‐up kinetic studies were conducted on the opening/closure of ion channels formed by the most abundant form of the SRs, syringo