Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer’s Patches of the Rat Intestine. / Fedorova, A.A.; Rybalchenko, O.V.; Okorokova, L.S.; Kapustina, V.V.; Orlova, O.G.; Markov, A.G.
в: Bulletin of Experimental Biology and Medicine, Том 177, 01.10.2024, стр. 757-762.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer’s Patches of the Rat Intestine
AU - Fedorova, A.A.
AU - Rybalchenko, O.V.
AU - Okorokova, L.S.
AU - Kapustina, V.V.
AU - Orlova, O.G.
AU - Markov, A.G.
N1 - Export Date: 10 November 2024 CODEN: BEXBA
PY - 2024/10/1
Y1 - 2024/10/1
N2 - To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer’s patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers’ patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa. © Springer Science+Business Media, LLC, part of Springer Nature 2024.
AB - To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer’s patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers’ patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa. © Springer Science+Business Media, LLC, part of Springer Nature 2024.
KW - lipopolysaccharides
KW - Peyer’s patches
KW - tight junctions
KW - tissue barriers
KW - vesicular transport
U2 - 10.1007/s10517-024-06263-0
DO - 10.1007/s10517-024-06263-0
M3 - статья
VL - 177
SP - 757
EP - 762
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
ER -
ID: 127213524