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Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer’s Patches of the Rat Intestine. / Fedorova, A.A.; Rybalchenko, O.V.; Okorokova, L.S.; Kapustina, V.V.; Orlova, O.G.; Markov, A.G.

в: Bulletin of Experimental Biology and Medicine, Том 177, 01.10.2024, стр. 757-762.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

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@article{5a13c5d31f104a0db1b4d32f82bc65b7,
title = "Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer{\textquoteright}s Patches of the Rat Intestine",
abstract = "To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer{\textquoteright}s patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers{\textquoteright} patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa. {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2024.",
keywords = "lipopolysaccharides, Peyer{\textquoteright}s patches, tight junctions, tissue barriers, vesicular transport",
author = "A.A. Fedorova and O.V. Rybalchenko and L.S. Okorokova and V.V. Kapustina and O.G. Orlova and A.G. Markov",
note = "Export Date: 10 November 2024 CODEN: BEXBA",
year = "2024",
month = oct,
day = "1",
doi = "10.1007/s10517-024-06263-0",
language = "Английский",
volume = "177",
pages = "757--762",
journal = "Bulletin of Experimental Biology and Medicine",
issn = "0007-4888",
publisher = "Springer Nature",

}

RIS

TY - JOUR

T1 - Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer’s Patches of the Rat Intestine

AU - Fedorova, A.A.

AU - Rybalchenko, O.V.

AU - Okorokova, L.S.

AU - Kapustina, V.V.

AU - Orlova, O.G.

AU - Markov, A.G.

N1 - Export Date: 10 November 2024 CODEN: BEXBA

PY - 2024/10/1

Y1 - 2024/10/1

N2 - To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer’s patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers’ patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa. © Springer Science+Business Media, LLC, part of Springer Nature 2024.

AB - To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer’s patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers’ patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa. © Springer Science+Business Media, LLC, part of Springer Nature 2024.

KW - lipopolysaccharides

KW - Peyer’s patches

KW - tight junctions

KW - tissue barriers

KW - vesicular transport

U2 - 10.1007/s10517-024-06263-0

DO - 10.1007/s10517-024-06263-0

M3 - статья

VL - 177

SP - 757

EP - 762

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

SN - 0007-4888

ER -

ID: 127213524