TY - JOUR

T1 - Ceruloplasmin decreases respiratory burst reaction during pregnancy

AU - Varfolomeeva, E.Y.

AU - Semenova, E.V.

AU - Sokolov, A.V.

AU - Aplin, K.D.

AU - Timofeeva, K.E.

AU - Vasilyev, V.B.

AU - Filatov, M.V.

PY - 2016

Y1 - 2016

N2 - Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin - a copper-containing glycoprotein - is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst rea

AB - Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin - a copper-containing glycoprotein - is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst rea

KW - Ceruloplasmin

KW - flow cytometry

KW - neutrophils

KW - pregnancy

KW - respiratory burst reaction

U2 - 10.1080/10715762.2016.1197395

DO - 10.1080/10715762.2016.1197395

M3 - Article

VL - 50

SP - 909

EP - 919

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 8

ER -