TY - JOUR

T1 - Biomolecular Condensates: Structure, Functions, Methods of Research

AU - Gorsheneva, Natalia A.

AU - Sopova, Julia V.

AU - Azarov, Vladimir V.

AU - Grizel, Anastasia V.

AU - Rubel, Aleksandr A.

N1 - Gorsheneva N.A., Sopova J.V., Azarov V.V., Grizel A.V., Rubel A.A. Biomolecular Condensates: Structure, Functions, Methods of Research. Biochemistry Moscow 89 (Suppl 1), S205–S223 (2024). https://doi.org/10.1134/S0006297924140116

PY - 2024/3/8

Y1 - 2024/3/8

N2 - Abstract: The term “biomolecular condensates” is used to describe membraneless compartments in eukaryotic cells, accumulating proteins and nucleic acids. Biomolecular condensates are formed as a result of liquid–liquid phase separation (LLPS). Often, they demonstrate properties of liquid-like droplets or gel-like aggregates; however, some of them may appear to have a more complex structure and high-order organization. Membraneless microcompartments are involved in diverse processes both in cytoplasm and in nucleus, among them ribosome biogenesis, regulation of gene expression, cell signaling, and stress response. Condensates properties and structure could be highly dynamic and are affected by various internal and external factors, e.g., concentration and interactions of components, solution temperature, pH, osmolarity, etc. In this review, we discuss variety of biomolecular condensates and their functions in live cells, describe their structure variants, highlight domain and primary sequence organization of the constituent proteins and nucleic acids. Finally, we describe current advances in methods that characterize structure, properties, morphology, and dynamics of biomolecular condensates in vitro and in vivo.

AB - Abstract: The term “biomolecular condensates” is used to describe membraneless compartments in eukaryotic cells, accumulating proteins and nucleic acids. Biomolecular condensates are formed as a result of liquid–liquid phase separation (LLPS). Often, they demonstrate properties of liquid-like droplets or gel-like aggregates; however, some of them may appear to have a more complex structure and high-order organization. Membraneless microcompartments are involved in diverse processes both in cytoplasm and in nucleus, among them ribosome biogenesis, regulation of gene expression, cell signaling, and stress response. Condensates properties and structure could be highly dynamic and are affected by various internal and external factors, e.g., concentration and interactions of components, solution temperature, pH, osmolarity, etc. In this review, we discuss variety of biomolecular condensates and their functions in live cells, describe their structure variants, highlight domain and primary sequence organization of the constituent proteins and nucleic acids. Finally, we describe current advances in methods that characterize structure, properties, morphology, and dynamics of biomolecular condensates in vitro and in vivo.

KW - LLPS

KW - биоконденсаты

KW - немембранные органеллы

KW - внутренне неупорядоченные области

KW - LLPS

KW - biomolecular condensates

KW - intrinsically disordered regions

KW - membraneless compartments

UR - https://link.springer.com/article/10.1134/S0006297924140116

UR - https://www.mendeley.com/catalogue/78267fdf-575c-3d1e-9c20-c3bf6c8e355b/

U2 - 10.1134/s0006297924140116

DO - 10.1134/s0006297924140116

M3 - Review article

VL - 89

SP - S205-S223

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

SN - 0006-2979

IS - suppl 1

ER -