Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Biological evaluation and molecular dynamics simulation of water-soluble fullerene derivative C60[C(COOH)2]3. / Gaponenko, Ivan N.; Ageev, Sergei V.; Iurev, Gleb O.; Shemchuk, Olga S.; Meshcheriakov, Anatolii A.; Petrov, Andrey V.; Solovtsova, Irina L.; Vasina, Lubov V.; Tennikova, Tatiana B.; Murin, Igor V.; Semenov, Konstantin N.; Sharoyko, Vladimir V.
в: Toxicology in Vitro, Том 62, 104683, 02.2020.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Biological evaluation and molecular dynamics simulation of water-soluble fullerene derivative C60[C(COOH)2]3
AU - Gaponenko, Ivan N.
AU - Ageev, Sergei V.
AU - Iurev, Gleb O.
AU - Shemchuk, Olga S.
AU - Meshcheriakov, Anatolii A.
AU - Petrov, Andrey V.
AU - Solovtsova, Irina L.
AU - Vasina, Lubov V.
AU - Tennikova, Tatiana B.
AU - Murin, Igor V.
AU - Semenov, Konstantin N.
AU - Sharoyko, Vladimir V.
PY - 2020/2
Y1 - 2020/2
N2 - One of the most studied fullerene members, C60, has a potential of application in various fields of biomedicine including reactive oxygen species (ROS) scavenging activity, inhibiting of tumours development, inactivating of viruses and bacteria, as well as elaboration of diagnostic and targeted drug delivery tools. However, the hydrophobicity of this molecule impedes its practical use, therefore the actuality of the research devoted to functionalisation of fullerenes leading to amphiphilic derivatives remains important. In this work, the water-soluble carboxylated fullerene derivative C60[C(COOH)2]3 was studied. Extensive biomedical investigation of this compound, namely, the binding with human serum albumin (HSA), radical scavenging activity in the reaction with diphenylpicrylhydrazyl (DPPH) radical, photodynamic properties, cytotoxicity in human embryonic kidney (HEK293) cell line, erythrocytes' haemolysis, platelet aggregation, and genotoxicity in human peripheral mononuclear cells (PBMC) was conducted. Moreover, the dynamic and structural characteristics of C60[C(COOH)2]3–H2O binary system were obtained using molecular dynamic (MD) method, and size distribution of C60[C(COOH)2]3 associates was measured.
AB - One of the most studied fullerene members, C60, has a potential of application in various fields of biomedicine including reactive oxygen species (ROS) scavenging activity, inhibiting of tumours development, inactivating of viruses and bacteria, as well as elaboration of diagnostic and targeted drug delivery tools. However, the hydrophobicity of this molecule impedes its practical use, therefore the actuality of the research devoted to functionalisation of fullerenes leading to amphiphilic derivatives remains important. In this work, the water-soluble carboxylated fullerene derivative C60[C(COOH)2]3 was studied. Extensive biomedical investigation of this compound, namely, the binding with human serum albumin (HSA), radical scavenging activity in the reaction with diphenylpicrylhydrazyl (DPPH) radical, photodynamic properties, cytotoxicity in human embryonic kidney (HEK293) cell line, erythrocytes' haemolysis, platelet aggregation, and genotoxicity in human peripheral mononuclear cells (PBMC) was conducted. Moreover, the dynamic and structural characteristics of C60[C(COOH)2]3–H2O binary system were obtained using molecular dynamic (MD) method, and size distribution of C60[C(COOH)2]3 associates was measured.
KW - carboxylated fullerene
KW - cytotoxicity
KW - genotoxicity
KW - radical scavenging activity
KW - haemolysis
KW - platelet aggregation
KW - Carboxylated fullerene
KW - Cytotoxicity
KW - Genotoxicity
KW - Haemolysis
KW - Platelet aggregation
KW - Radical scavenging activity
KW - Water
KW - Free Radical Scavengers/pharmacology
KW - Biphenyl Compounds/toxicity
KW - Humans
KW - Solubility
KW - Male
KW - Cell Survival/drug effects
KW - Molecular Dynamics Simulation
KW - Picrates/toxicity
KW - Computer Simulation
KW - HEK293 Cells
KW - Protein Binding
KW - Adult
KW - Female
KW - Platelet Aggregation/drug effects
KW - Fullerenes/chemistry
KW - Mutagens/toxicity
KW - DNA-DAMAGE
KW - NANOMATERIALS
KW - DELIVERY
KW - PHYSICOCHEMICAL PROPERTIES
KW - C-60
KW - CARBOXYFULLERENE
KW - CELLS
KW - OXIDATIVE STRESS
KW - SINGLET OXYGEN
KW - SYSTEMS
UR - http://www.scopus.com/inward/record.url?scp=85074150018&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/9b7b7431-17a7-3aea-92ec-fda343a59a66/
U2 - 10.1016/j.tiv.2019.104683
DO - 10.1016/j.tiv.2019.104683
M3 - Article
C2 - 31639450
AN - SCOPUS:85074150018
VL - 62
JO - Toxicology in Vitro
JF - Toxicology in Vitro
SN - 0887-2333
M1 - 104683
ER -
ID: 50969182