Bio-inspired amphiphilic block-copolymers based on synthetic glycopolymer and poly(Amino acid) as potential drug delivery systems

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Bio-inspired amphiphilic block-copolymers based on synthetic glycopolymer and poly(Amino acid) as potential drug delivery systems. / Levit, Mariia ; Zashikhina, Natalia ; Vdovchenko, Alena ; Dobrodumov, Anatoliy ; Zakharova, Natalya ; Kashina , Anna ; Rühl, Eckart ; Lavrentieva , Antonina; Scheper , Thomas ; Tennikova, Tatiana ; Korzhikova-Vlakh, Evgenia .

в: Polymers, Том 12, № 1, 183, 01.2020, стр. 1-27.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{10668acd44324895af25ff34a756b548,

title = "Bio-inspired amphiphilic block-copolymers based on synthetic glycopolymer and poly(Amino acid) as potential drug delivery systems",

abstract = "In this work, a method to prepare hybrid amphiphilic block copolymers consisting of biocompatible synthetic glycopolymer with non-degradable backbone and biodegradable poly(amino acid) (PAA) was developed. The glycopolymer, poly(2-deoxy-2-methacrylamido-D-glucose) (PMAG), was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Two methods for modifying the terminal dithiobenzoate-group of PMAG was investigated to obtain the macroinitiator bearing a primary aliphatic amino group, which is required for ring-opening polymerization of N-carboxyanhydrides of hydrophobic α-amino acids. The synthesized amphiphilic block copolymers were carefully analyzed using a set of different physico-chemical methods to establish their composition and molecular weight. The developed amphiphilic copolymers tended to self-assemble in nanoparticles of different morphology that depended on the nature of the hydrophobic amino acid present in the copolymer. The hydrodynamic diameter, morphology, and cytotoxicity of polymer particles based on PMAG-b-PAA were evaluated using dynamic light scattering (DLS) and transmission electron microscopy (TEM), as well as CellTiter-Blue (CTB) assay, respectively. The redox-responsive properties of nanoparticles were evaluated in the presence of glutathione taken at different concentrations. Moreover, the encapsulation of paclitaxel into PMAG-b-PAA particles and their cytotoxicity on human lung carcinoma cells (A549) and human breast adenocarcinoma cells (MCF-7) were studied.",

keywords = "Amphiphilic block copolymers, Drug delivery systems, Modification of terminal groups, Nanoparticles, Paclitaxel, Poly(amino acids), Redox-responsive systems, Synthetic glycopolymers, POLYMERS, drug delivery systems, paclitaxel, VESICLES, poly(amino acids), MICELLES, POLYMERIZATION, modification of terminal groups, amphiphilic block copolymers, redox-responsive systems, synthetic glycopolymers, FUNCTIONALIZATION, NANOPARTICLES, nanoparticles, RAFT, Poly(vinylidene fluoride), Blend, Physical properties, Nanocomposites, Polyamide 6",

author = "Mariia Levit and Natalia Zashikhina and Alena Vdovchenko and Anatoliy Dobrodumov and Natalya Zakharova and Anna Kashina and Eckart R{\"u}hl and Antonina Lavrentieva and Thomas Scheper and Tatiana Tennikova and Evgenia Korzhikova-Vlakh",

year = "2020",

month = jan,

doi = "10.3390/polym12010183",

language = "English",

volume = "12",

pages = "1--27",

journal = "Polymers",

issn = "2073-4360",

publisher = "MDPI AG",

number = "1",

}

RIS

TY - JOUR

T1 - Bio-inspired amphiphilic block-copolymers based on synthetic glycopolymer and poly(Amino acid) as potential drug delivery systems

AU - Levit, Mariia

AU - Zashikhina, Natalia

AU - Vdovchenko, Alena

AU - Dobrodumov, Anatoliy

AU - Zakharova, Natalya

AU - Kashina , Anna

AU - Rühl, Eckart

AU - Lavrentieva , Antonina

AU - Scheper , Thomas

AU - Tennikova, Tatiana

AU - Korzhikova-Vlakh, Evgenia

PY - 2020/1

Y1 - 2020/1

N2 - In this work, a method to prepare hybrid amphiphilic block copolymers consisting of biocompatible synthetic glycopolymer with non-degradable backbone and biodegradable poly(amino acid) (PAA) was developed. The glycopolymer, poly(2-deoxy-2-methacrylamido-D-glucose) (PMAG), was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Two methods for modifying the terminal dithiobenzoate-group of PMAG was investigated to obtain the macroinitiator bearing a primary aliphatic amino group, which is required for ring-opening polymerization of N-carboxyanhydrides of hydrophobic α-amino acids. The synthesized amphiphilic block copolymers were carefully analyzed using a set of different physico-chemical methods to establish their composition and molecular weight. The developed amphiphilic copolymers tended to self-assemble in nanoparticles of different morphology that depended on the nature of the hydrophobic amino acid present in the copolymer. The hydrodynamic diameter, morphology, and cytotoxicity of polymer particles based on PMAG-b-PAA were evaluated using dynamic light scattering (DLS) and transmission electron microscopy (TEM), as well as CellTiter-Blue (CTB) assay, respectively. The redox-responsive properties of nanoparticles were evaluated in the presence of glutathione taken at different concentrations. Moreover, the encapsulation of paclitaxel into PMAG-b-PAA particles and their cytotoxicity on human lung carcinoma cells (A549) and human breast adenocarcinoma cells (MCF-7) were studied.

AB - In this work, a method to prepare hybrid amphiphilic block copolymers consisting of biocompatible synthetic glycopolymer with non-degradable backbone and biodegradable poly(amino acid) (PAA) was developed. The glycopolymer, poly(2-deoxy-2-methacrylamido-D-glucose) (PMAG), was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Two methods for modifying the terminal dithiobenzoate-group of PMAG was investigated to obtain the macroinitiator bearing a primary aliphatic amino group, which is required for ring-opening polymerization of N-carboxyanhydrides of hydrophobic α-amino acids. The synthesized amphiphilic block copolymers were carefully analyzed using a set of different physico-chemical methods to establish their composition and molecular weight. The developed amphiphilic copolymers tended to self-assemble in nanoparticles of different morphology that depended on the nature of the hydrophobic amino acid present in the copolymer. The hydrodynamic diameter, morphology, and cytotoxicity of polymer particles based on PMAG-b-PAA were evaluated using dynamic light scattering (DLS) and transmission electron microscopy (TEM), as well as CellTiter-Blue (CTB) assay, respectively. The redox-responsive properties of nanoparticles were evaluated in the presence of glutathione taken at different concentrations. Moreover, the encapsulation of paclitaxel into PMAG-b-PAA particles and their cytotoxicity on human lung carcinoma cells (A549) and human breast adenocarcinoma cells (MCF-7) were studied.

KW - Amphiphilic block copolymers

KW - Drug delivery systems

KW - Modification of terminal groups

KW - Nanoparticles

KW - Paclitaxel

KW - Poly(amino acids)

KW - Redox-responsive systems

KW - Synthetic glycopolymers

KW - POLYMERS

KW - drug delivery systems

KW - paclitaxel

KW - VESICLES

KW - poly(amino acids)

KW - MICELLES

KW - POLYMERIZATION

KW - modification of terminal groups

KW - amphiphilic block copolymers

KW - redox-responsive systems

KW - synthetic glycopolymers

KW - FUNCTIONALIZATION

KW - NANOPARTICLES

KW - nanoparticles

KW - RAFT

KW - Poly(vinylidene fluoride)

KW - Blend

KW - Physical properties

KW - Nanocomposites

KW - Polyamide 6

UR - http://www.scopus.com/inward/record.url?scp=85082653219&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/30c0410f-d426-3ad6-81d3-91b9bd181876/

U2 - 10.3390/polym12010183

DO - 10.3390/polym12010183

M3 - Article

AN - SCOPUS:85082653219

VL - 12

SP - 1

EP - 27

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 1

M1 - 183

ER -

ID: 61444641