Representing the most important part of the intestinal immune system, Peyer’s patches (PP) form an aggregation of lymphocytes, macrophages and dendritic cells located just underneath the basal layer of the intestinal epithelium. The follicle-associated epithelium (FAE) contains specific cells (microfold cells) for antigen uptake and transport through the epithelial layer to the underlying PP immune cells. Therefore interactions with the immune system are possible. Since a tight intestinal barrier is a prerequisite for this function, we questioned whether in porcine intestine there are functional and molecular differences of tight junctions in comparison to villous epithelium