TY - JOUR

T1 - Bactericidal effect of a dielectric barrier discharge plasma jet generated in laminar and preturbulent helium flows

AU - Stepanova, Olga

AU - Rybalchenko, Oksana

AU - Pinchuk, Mikhail

AU - Astafiev, Alexander

AU - Orlova, Olga

AU - Spodobin, Valentin

AU - Kudryavtsev, Anatoly

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The bactericidal effect of a helium dielectric barrier discharge plasma jet generated in laminar (at 3 m/s) and preturbulent (at 10 m/s) helium flows is considered in terms of average discharge power and distance of treatment of Escherichia coli cells grown on a nutrient agar surface in a Petri dish. Sizes of bacteria inhibition zones are estimated and mor-phological changes in ultrastructure of bacterial cells are revealed using electron microscopy. This study suggests that the gas flow regime is an important factor in the production of the helium plasma jet used to inhibit bacteria. We reveal the different responses of E. coli cells treated by the helium plasma jet generated in laminar and preturbulent gas flows. Numerous focal destructions appear in the cells after 2 min of treatment with the laminar gas flow regime. Along with these focal destructions, expansion of cytoplasm and a superhelix of nucleoids are revealed with the preturbulent regime. We explain differences in behavior of cell morphology under the plasmajet formed at different gas flow regimes by the different gas-dynamic conditions ofmixing ambient air into the helium flow.

AB - The bactericidal effect of a helium dielectric barrier discharge plasma jet generated in laminar (at 3 m/s) and preturbulent (at 10 m/s) helium flows is considered in terms of average discharge power and distance of treatment of Escherichia coli cells grown on a nutrient agar surface in a Petri dish. Sizes of bacteria inhibition zones are estimated and mor-phological changes in ultrastructure of bacterial cells are revealed using electron microscopy. This study suggests that the gas flow regime is an important factor in the production of the helium plasma jet used to inhibit bacteria. We reveal the different responses of E. coli cells treated by the helium plasma jet generated in laminar and preturbulent gas flows. Numerous focal destructions appear in the cells after 2 min of treatment with the laminar gas flow regime. Along with these focal destructions, expansion of cytoplasm and a superhelix of nucleoids are revealed with the preturbulent regime. We explain differences in behavior of cell morphology under the plasmajet formed at different gas flow regimes by the different gas-dynamic conditions ofmixing ambient air into the helium flow.

KW - Bactericidal effect

KW - Dielectric barrier discharge

KW - Electron microscopy

KW - Helium plasmajet

KW - Laminar gas flow

KW - Morphological properties

KW - Preturbulent gas flow

UR - http://www.scopus.com/inward/record.url?scp=85041857994&partnerID=8YFLogxK

U2 - 10.1615/PlasmaMed.2017019064

DO - 10.1615/PlasmaMed.2017019064

M3 - Article

AN - SCOPUS:85041857994

VL - 7

SP - 187

EP - 200

JO - Plasma Medicine

JF - Plasma Medicine

SN - 1947-5764

IS - 3

ER -