TY - JOUR

T1 - Bacterial Effect on the Crystallization of Mineral Phases in a Solution Simulating Human Urine

AU - Франк-Каменецкая, Ольга Викторовна

AU - Изатулина, Алина Ростамовна

AU - Николаев, Антон Михайлович

AU - Кузьмина, Мария Анатольевна

AU - Малышев, Владимир Васильевич

PY - 2019/5/19

Y1 - 2019/5/19

N2 - The effect of bacteria that present in the human urine (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus) was studied under the conditions of biomimetic synthesis. It was shown that the addition of bacteria significantly affects both the phase composition of the synthesized material and the position of crystallization boundaries of the resulting phosphate phases, which can shift toward more acidic (struvite, apatite) or toward more alkaline (brushite) conditions. Under conditions of oxalate mineralization, bacteria accelerate the nucleation of calcium oxalates by almost two times and also increase the amount of oxalate precipitates along with phosphates and stabilize the calcium oxalate dihydrate (weddellite). The multidirectional changes in the pH values of the solutions, which are the result of the interaction of all system components and the crystallization process, were analyzed. The obtained results are the scientific basis for understanding the mechanisms of bacterial involvement in stone formation within the human body and the creation of biotechnological methods that inhibit this process.

AB - The effect of bacteria that present in the human urine (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus) was studied under the conditions of biomimetic synthesis. It was shown that the addition of bacteria significantly affects both the phase composition of the synthesized material and the position of crystallization boundaries of the resulting phosphate phases, which can shift toward more acidic (struvite, apatite) or toward more alkaline (brushite) conditions. Under conditions of oxalate mineralization, bacteria accelerate the nucleation of calcium oxalates by almost two times and also increase the amount of oxalate precipitates along with phosphates and stabilize the calcium oxalate dihydrate (weddellite). The multidirectional changes in the pH values of the solutions, which are the result of the interaction of all system components and the crystallization process, were analyzed. The obtained results are the scientific basis for understanding the mechanisms of bacterial involvement in stone formation within the human body and the creation of biotechnological methods that inhibit this process.

KW - Apatite

KW - Biomimetic synthesis

KW - Brushite

KW - Calcium oxalate

KW - Escherichia coli

KW - Klebsiella pneumoniae

KW - Octocalcium phosphate

KW - Pathogen crystallization

KW - Pseudomonas aeruginosa

KW - Renal stone

KW - Staphylococcus aureus

KW - Struvite

KW - Whitlockite

UR - http://www.scopus.com/inward/record.url?scp=85073286444&partnerID=8YFLogxK

U2 - 10.3390/cryst9050259

DO - 10.3390/cryst9050259

M3 - Article

VL - 9,

JO - Liquid Crystals Today

JF - Liquid Crystals Today

SN - 1358-314X

IS - 5

M1 - 259

ER -