Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive promoter

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Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive promoter. / Pozhvanov, G. A.; Medvedev, S. S.

в: Russian Journal of Plant Physiology, Том 55, № 5, 01.09.2008, стр. 706-711.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{442e73bc6a83433588629442f4047560,

title = "Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive promoter",

abstract = "To visualize phytohormone localization in plant tissues, transgenic plants comprising the GUS reporter gene are often used. However, until now only qualitative assessment of the hormone presence was available. In this work, we suggested the method for IAA quantification in transgenic DR5::GUS Arabidopsis thaliana L. plants by the analysis of digital images. An empirical quadratic dependence was established between the IAA concentration in medium and the level of GUS-dependent staining. Using this method, we demonstrated that, after A. thaliana root gravistimulation for 90 min, auxin lateral redistribution occurred. It resulted in the increase in the IAA concentration in the lower root part (in the elongation zone and apical meristem) by 200% on the average.",

keywords = "Arabidopsis thaliana, Digital image color analysis, Gravitropism, GUS reporter gene, Histochemical staining, IAA",

author = "Pozhvanov, {G. A.} and Medvedev, {S. S.}",

year = "2008",

month = sep,

day = "1",

doi = "10.1134/S1021443708050166",

language = "English",

volume = "55",

pages = "706--711",

journal = "Russian Journal of Plant Physiology",

issn = "1021-4437",

publisher = "Pleiades Publishing",

number = "5",

}

RIS

TY - JOUR

T1 - Auxin quantification based on histochemical staining of GUS under the control of auxin-responsive promoter

AU - Pozhvanov, G. A.

AU - Medvedev, S. S.

PY - 2008/9/1

Y1 - 2008/9/1

N2 - To visualize phytohormone localization in plant tissues, transgenic plants comprising the GUS reporter gene are often used. However, until now only qualitative assessment of the hormone presence was available. In this work, we suggested the method for IAA quantification in transgenic DR5::GUS Arabidopsis thaliana L. plants by the analysis of digital images. An empirical quadratic dependence was established between the IAA concentration in medium and the level of GUS-dependent staining. Using this method, we demonstrated that, after A. thaliana root gravistimulation for 90 min, auxin lateral redistribution occurred. It resulted in the increase in the IAA concentration in the lower root part (in the elongation zone and apical meristem) by 200% on the average.

AB - To visualize phytohormone localization in plant tissues, transgenic plants comprising the GUS reporter gene are often used. However, until now only qualitative assessment of the hormone presence was available. In this work, we suggested the method for IAA quantification in transgenic DR5::GUS Arabidopsis thaliana L. plants by the analysis of digital images. An empirical quadratic dependence was established between the IAA concentration in medium and the level of GUS-dependent staining. Using this method, we demonstrated that, after A. thaliana root gravistimulation for 90 min, auxin lateral redistribution occurred. It resulted in the increase in the IAA concentration in the lower root part (in the elongation zone and apical meristem) by 200% on the average.

KW - Arabidopsis thaliana

KW - Digital image color analysis

KW - Gravitropism

KW - GUS reporter gene

KW - Histochemical staining

KW - IAA

UR - http://www.scopus.com/inward/record.url?scp=56749154582&partnerID=8YFLogxK

U2 - 10.1134/S1021443708050166

DO - 10.1134/S1021443708050166

M3 - Article

AN - SCOPUS:56749154582

VL - 55

SP - 706

EP - 711

JO - Russian Journal of Plant Physiology

JF - Russian Journal of Plant Physiology

SN - 1021-4437

IS - 5

ER -

ID: 36021923