TY - JOUR

T1 - Automated liquid–liquid deep eutectic solvents based microextraction procedure for determination of acrylamide in foodstuffs by high-performance liquid chromatography with ultraviolet detection

AU - Шишов, Андрей Юрьевич

AU - Маркова, Ульяна Олеговна

AU - Давыдова, Екатерина Геннадьевна

AU - Булатов, Андрей Васильевич

PY - 2024/12

Y1 - 2024/12

N2 - For the rapid and efficient determination of acrylamide in food products by HPLC-UV, an environmentally friendly analytical approach has been developed, including liquid-phase extraction and subsequent liquid–liquid microextraction using a deep eutectic solvent. To automate the procedure, the flow-through “lab in a syringe” method was used. Acrylamide is considered a potential endocrine disrupting chemical and its main source is fried foods, which are widely consumed by both children and adults. To extract and concentrate acrylamide, hydrophobic deep-eutectic solvents based on various carboxylic acids and natural terpenoids were studied for the first time. It was found that benzoic acid, as a precursor of the extraction solvent, promotes the transfer of hydrophilic acrylamide from the aqueous phase of the sample due to the interaction of carboxyl and amide groups. The procedure has been validated and used effectively to estimate acrylamide content in beetroot and corn chips. Under optimal conditions, the detection limit was 0.01 mg/kg. Unlike existing methods, the proposed method is fully automated, does not require hazardous organic solvents and additional derivatization stages, and at the same time allows the determination of acrylamide at a level below established standards.

AB - For the rapid and efficient determination of acrylamide in food products by HPLC-UV, an environmentally friendly analytical approach has been developed, including liquid-phase extraction and subsequent liquid–liquid microextraction using a deep eutectic solvent. To automate the procedure, the flow-through “lab in a syringe” method was used. Acrylamide is considered a potential endocrine disrupting chemical and its main source is fried foods, which are widely consumed by both children and adults. To extract and concentrate acrylamide, hydrophobic deep-eutectic solvents based on various carboxylic acids and natural terpenoids were studied for the first time. It was found that benzoic acid, as a precursor of the extraction solvent, promotes the transfer of hydrophilic acrylamide from the aqueous phase of the sample due to the interaction of carboxyl and amide groups. The procedure has been validated and used effectively to estimate acrylamide content in beetroot and corn chips. Under optimal conditions, the detection limit was 0.01 mg/kg. Unlike existing methods, the proposed method is fully automated, does not require hazardous organic solvents and additional derivatization stages, and at the same time allows the determination of acrylamide at a level below established standards.

KW - Acrylamide

KW - Deep eutectic solvent

KW - Flow analysis

KW - Foodstuffs

KW - High-performance liquid chromatography with UV detection

UR - https://www.mendeley.com/catalogue/6f1f6a52-832b-3b50-b81c-634ee0456fde/

U2 - 10.1007/s13197-024-05999-x

DO - 10.1007/s13197-024-05999-x

M3 - Article

C2 - 39431190

VL - 61

SP - 2332

EP - 2342

JO - Journal of Food Science and Technology

JF - Journal of Food Science and Technology

SN - 0022-1155

IS - 12

ER -