TY - JOUR

T1 - Application of Natural Deep Eutectic Solvents for Extraction of Bioactive Components from Rhodiola rosea ( L .)

AU - Цветов, Никита

AU - Паукшта, Оксана

AU - Фокина, Надежда

AU - Володина, Наталья Юрьевна

AU - Самаров, Артемий Андреевич

PY - 2023/1/16

Y1 - 2023/1/16

N2 - Rhodiola rosea (L.) is a valuable source of nutrients. Nutrients have adaptogenic, immunostimulating, nootropic, anti-inflammatory and anti-cancer properties. Natural deep eutectic solvents (NADES) consisting of choline chloride and malonic, malic, tartaric or citric acids have been first used to extract biologically active substances from R. rosea. The total content of polyphenols has been determined by the Folin-Ciocalteu method for all extracts. Antioxidant activity has been determined by the phosphomolybdate method, and antiradical activity has been determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Rosavin concentration has been determined by high-performance liquid chromatography (HPLC). Extraction kinetics has been evaluated regarding the effectiveness of NADES with each other and with reference solvents (water and 50% ethanol) has been made. Extraction conditions have been optimized according to the Box-Behnken design of the experiment. The optimal parameters of the extraction process have been established. The antibacterial activity of NADES-based extracts against bacterial cultures of Micrococcus luteus, Pseudomonas fluorescens, and Bacillus subtilis has been studied.

AB - Rhodiola rosea (L.) is a valuable source of nutrients. Nutrients have adaptogenic, immunostimulating, nootropic, anti-inflammatory and anti-cancer properties. Natural deep eutectic solvents (NADES) consisting of choline chloride and malonic, malic, tartaric or citric acids have been first used to extract biologically active substances from R. rosea. The total content of polyphenols has been determined by the Folin-Ciocalteu method for all extracts. Antioxidant activity has been determined by the phosphomolybdate method, and antiradical activity has been determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Rosavin concentration has been determined by high-performance liquid chromatography (HPLC). Extraction kinetics has been evaluated regarding the effectiveness of NADES with each other and with reference solvents (water and 50% ethanol) has been made. Extraction conditions have been optimized according to the Box-Behnken design of the experiment. The optimal parameters of the extraction process have been established. The antibacterial activity of NADES-based extracts against bacterial cultures of Micrococcus luteus, Pseudomonas fluorescens, and Bacillus subtilis has been studied.

KW - Rhodiola rosea (L.)

KW - antibacterial activity

KW - natural deep eutectic solvents

KW - ultrasound-assisted extraction

UR - https://www.mendeley.com/catalogue/4c3ff13b-4e6d-36de-882a-2f2707ffa889/

U2 - 10.3390/molecules28020912

DO - 10.3390/molecules28020912

M3 - Article

C2 - 36677969

VL - 28

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 2

M1 - 912

ER -