Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Analysis of NAD and NAD-Dependent Protein Deacetylation in Mouse Tissues. / Solovjeva, L. V.; Panchenko, A. V.; Shabalin, K. A.; Nerinovski, K. B.; Yakimov, A. P.; Gubareva, E. A.; Svetlova, M. P.; Mudrak, O. S.; Khodorkovskiy, M. A.; Nikiforov, A. A.; Kulikova, V. A.
в: Cell and Tissue Biology, Том 12, № 6, 01.11.2018, стр. 491-495.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Analysis of NAD and NAD-Dependent Protein Deacetylation in Mouse Tissues
AU - Solovjeva, L. V.
AU - Panchenko, A. V.
AU - Shabalin, K. A.
AU - Nerinovski, K. B.
AU - Yakimov, A. P.
AU - Gubareva, E. A.
AU - Svetlova, M. P.
AU - Mudrak, O. S.
AU - Khodorkovskiy, M. A.
AU - Nikiforov, A. A.
AU - Kulikova, V. A.
N1 - Funding Information: ACKNOWLEDGMENTS This work was financially supported by the Russian Science Foundation, project no. 16-14-10240. Publisher Copyright: © 2018, Pleiades Publishing, Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Abstract—: Nicotinamide adenine dinucleotide (NAD) plays a key role in the vital metabolic and regulatory processes in mammals. Disturbance of the NAD level regulation is associated with the development of such serious diseases as pellagra, neurodegenerative and cardiovascular disorders, diabetes, cancer and others. This paper presents an experimental approach that allows to determine the amount of NAD+ in mouse tissues using NMR spectroscopy, as well as the level of NAD+-dependent deacetylation of proteins in the cytosol and mitochondria.
AB - Abstract—: Nicotinamide adenine dinucleotide (NAD) plays a key role in the vital metabolic and regulatory processes in mammals. Disturbance of the NAD level regulation is associated with the development of such serious diseases as pellagra, neurodegenerative and cardiovascular disorders, diabetes, cancer and others. This paper presents an experimental approach that allows to determine the amount of NAD+ in mouse tissues using NMR spectroscopy, as well as the level of NAD+-dependent deacetylation of proteins in the cytosol and mitochondria.
KW - deacetylation
KW - immunoblotting
KW - NAD
KW - NMR spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85059350433&partnerID=8YFLogxK
U2 - 10.31116/tsitol.2018.07.14
DO - 10.31116/tsitol.2018.07.14
M3 - Article
AN - SCOPUS:85059350433
VL - 12
SP - 491
EP - 495
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 6
ER -
ID: 73949256