TY - JOUR

T1 - An effervescence-assisted dispersive liquid–liquid microextraction based on deep eutectic solvent decomposition

T2 - Determination of ketoprofen and diclofenac in liver

AU - Shishov, Andrey

AU - Gerasimov, Artur

AU - Nechaeva, Daria

AU - Volodina, Natalia

AU - Bessonova, Elena

AU - Bulatov, Andrey

PY - 2020/7

Y1 - 2020/7

N2 - A simple and green procedure was proposed for the separation and preconcentration of ionizable analytes from complex solid-phase samples. The developed procedure was applied to the HPLC–MS/MS determination of two nonsteroidal anti-inflammatory drugs (ketoprofen and diclofenac) in beef liver samples. The procedure included two stages. The first one assumed separation of analytes from a liver sample in a sodium carbonate solution (pH 11). In this case analytes ionization promoted mass-transfer from solid sample phase to alkaline aqueous phase. The second one assumed an effervescence-assisted dispersive liquid–liquid microextraction based on deep eutectic solvent decomposition in alkaline aqueous sample phase resulting extragent and proton donor agent formation. As result, a simple effervescent reaction between proton donor agent and effervescent agent (sodium carbonate) produced carbon dioxide and assisted in a dispersion of extragent. Deep eutectic solvent consisting of menthol and formic acid (1:40 mol) provided excellent extraction of ketoprofen and diclofenac. LODs calculated from a blank test, based on 3σ, were 0.1 and 0.3 μg kg−1 for ketoprofen and diclofenac, respectively.

AB - A simple and green procedure was proposed for the separation and preconcentration of ionizable analytes from complex solid-phase samples. The developed procedure was applied to the HPLC–MS/MS determination of two nonsteroidal anti-inflammatory drugs (ketoprofen and diclofenac) in beef liver samples. The procedure included two stages. The first one assumed separation of analytes from a liver sample in a sodium carbonate solution (pH 11). In this case analytes ionization promoted mass-transfer from solid sample phase to alkaline aqueous phase. The second one assumed an effervescence-assisted dispersive liquid–liquid microextraction based on deep eutectic solvent decomposition in alkaline aqueous sample phase resulting extragent and proton donor agent formation. As result, a simple effervescent reaction between proton donor agent and effervescent agent (sodium carbonate) produced carbon dioxide and assisted in a dispersion of extragent. Deep eutectic solvent consisting of menthol and formic acid (1:40 mol) provided excellent extraction of ketoprofen and diclofenac. LODs calculated from a blank test, based on 3σ, were 0.1 and 0.3 μg kg−1 for ketoprofen and diclofenac, respectively.

KW - Deep eutectic solvent

KW - Diclofenac

KW - Dispersive liquid-liquid microextraction

KW - HPLC–MS/MS

KW - Ketoprofen

KW - Liver

KW - Menthol

KW - Microextraction

KW - PRECONCENTRATION

KW - FUNGICIDES

KW - CHROMATOGRAPHY

KW - MASS-SPECTROMETRY

KW - EXTRACTION

KW - HPLC-MS/MS

KW - NONSTEROIDAL ANTIINFLAMMATORY DRUGS

KW - RESIDUES

KW - MILK

UR - http://www.scopus.com/inward/record.url?scp=85082202534&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/0660a3b3-fd16-318a-a551-ac449efbc138/

U2 - 10.1016/j.microc.2020.104837

DO - 10.1016/j.microc.2020.104837

M3 - Article

AN - SCOPUS:85082202534

VL - 156

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

M1 - 104837

ER -