Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
An automated in-syringe switchable hydrophilicity solvent-based microextraction. / Pochivalov, Aleksei ; Vakh, Christina ; Garmonov, Sergei; Moskvin, Leonid ; Bulatov, Andrey .
в: Talanta, Том 209, № 1, 120587, 01.03.2020.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - An automated in-syringe switchable hydrophilicity solvent-based microextraction
AU - Pochivalov, Aleksei
AU - Vakh, Christina
AU - Garmonov, Sergei
AU - Moskvin, Leonid
AU - Bulatov, Andrey
N1 - Publisher Copyright: © 2019 Elsevier B.V.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - A fully automated in-syringe switchable hydrophilicity solvent-based microextraction approach was suggested for the first time. Di-(2-ethylhexyl)phosphoric acid was investigated as a novel switchable hydrophilicity solvent. The microextraction procedure implemented into a syringe pump included dissociation of the extractant in alkaline sample solution resulting in homogeneous solution formation followed by in situ organic phase generation by acidification and its separation. The microextraction procedure was applied to the HPLC-UV determination of antimicrobial drugs (sulfamethoxazole and sulfamethazine) in human urine samples as a proof-of-concept example. The calibration graphs were linear over the concentration ranges of 0.06–50 mg L−1 for sulfamethoxazole and 0.13–50 mg L−1 for sulfamethazine. The LODs calculated from the blank tests based on 3σ were 0.02 and 0.04 mg L−1 for sulfamethoxazole and sulfamethazine, respectively. The sample throughput was 12 samples h−1. The possibility of using the proposed procedure for assessing sulfamethoxazole/sulfamethazine acetylation in metabolic processes by individual human phenotypes was shown.
AB - A fully automated in-syringe switchable hydrophilicity solvent-based microextraction approach was suggested for the first time. Di-(2-ethylhexyl)phosphoric acid was investigated as a novel switchable hydrophilicity solvent. The microextraction procedure implemented into a syringe pump included dissociation of the extractant in alkaline sample solution resulting in homogeneous solution formation followed by in situ organic phase generation by acidification and its separation. The microextraction procedure was applied to the HPLC-UV determination of antimicrobial drugs (sulfamethoxazole and sulfamethazine) in human urine samples as a proof-of-concept example. The calibration graphs were linear over the concentration ranges of 0.06–50 mg L−1 for sulfamethoxazole and 0.13–50 mg L−1 for sulfamethazine. The LODs calculated from the blank tests based on 3σ were 0.02 and 0.04 mg L−1 for sulfamethoxazole and sulfamethazine, respectively. The sample throughput was 12 samples h−1. The possibility of using the proposed procedure for assessing sulfamethoxazole/sulfamethazine acetylation in metabolic processes by individual human phenotypes was shown.
KW - Automated in-syringe switchable hydrophilicity solvent-based microextraction
KW - Flow analysis
KW - High performance liquid chromatography with ultraviolet detection
KW - Acetylation phenotype
KW - SULFONAMIDE
KW - Human urine
KW - Sulfonamides
UR - https://www.sciencedirect.com/science/article/pii/S0039914019312202
UR - https://www.ncbi.nlm.nih.gov/pubmed/31892021
UR - http://www.scopus.com/inward/record.url?scp=85075829200&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2019.120587
DO - 10.1016/j.talanta.2019.120587
M3 - Article
VL - 209
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 1
M1 - 120587
ER -
ID: 49184724