TY - JOUR

T1 - Amphiphilic Polypeptides Obtained by the Post-Polymerization Modification of Poly(Glutamic Acid) and Their Evaluation as Delivery Systems for Hydrophobic Drugs

AU - Джужа, Аполлинария Юрьевна

AU - Тарасенко, Ирина Ильинична

AU - Атаназе, Леонард

AU - Лаврентьева, Антонина

AU - Коржикова-Влах, Евгения Георгиевна

PY - 2023/1/5

Y1 - 2023/1/5

N2 - Synthetic poly(amino acids) are a unique class of macromolecules imitating natural polypeptides and are widely considered as carriers for drug and gene delivery. In this work, we synthesized, characterized and studied the properties of amphiphilic copolymers obtained by the post-polymerization modification of poly(α,L-glutamic acid) with various hydrophobic and basic L-amino acids and D-glucosamine. The resulting glycopolypeptides were capable of forming nanoparticles that exhibited reduced macrophage uptake and were non-toxic to human lung epithelial cells (BEAS-2B). Moreover, the developed nanoparticles were suitable for loading hydrophobic cargo. In particular, paclitaxel nanoformulations had a size of 170-330 nm and demonstrated a high cytostatic efficacy against human lung adenocarcinoma (A549). In general, the obtained nanoparticles were comparable in terms of their characteristics and properties to those based on amphiphilic (glyco)polypeptides obtained by copolymerization methods.

AB - Synthetic poly(amino acids) are a unique class of macromolecules imitating natural polypeptides and are widely considered as carriers for drug and gene delivery. In this work, we synthesized, characterized and studied the properties of amphiphilic copolymers obtained by the post-polymerization modification of poly(α,L-glutamic acid) with various hydrophobic and basic L-amino acids and D-glucosamine. The resulting glycopolypeptides were capable of forming nanoparticles that exhibited reduced macrophage uptake and were non-toxic to human lung epithelial cells (BEAS-2B). Moreover, the developed nanoparticles were suitable for loading hydrophobic cargo. In particular, paclitaxel nanoformulations had a size of 170-330 nm and demonstrated a high cytostatic efficacy against human lung adenocarcinoma (A549). In general, the obtained nanoparticles were comparable in terms of their characteristics and properties to those based on amphiphilic (glyco)polypeptides obtained by copolymerization methods.

KW - Amino Acids

KW - Drug Carriers/chemistry

KW - Drug Delivery Systems/methods

KW - Glutamic Acid

KW - Humans

KW - Nanoparticles/chemistry

KW - Peptides/chemistry

KW - Polymerization

KW - paclitaxel

KW - drug delivery

KW - amphiphilic poly(amino acids)

KW - polypeptides

KW - nanoparticles

KW - hydrophobic drugs

KW - post-polymerization modification

UR - https://www.mendeley.com/catalogue/f59dfba1-4dd2-311b-8379-1974abe68d35/

U2 - 10.3390/ijms24021049

DO - 10.3390/ijms24021049

M3 - Article

C2 - 36674566

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 2

M1 - 1049

ER -