TY - JOUR

T1 - Alteration of transport activity of proton pumps in coleoptile cells during early development stages of maize seedlings

AU - Shishova, M.F.

AU - Tankelyun, O.V.

AU - Rudashevskaya, E.L.

AU - Emel’yanov, V.V.

AU - Shakhova, N.V.

AU - Kirpichnikova, A.A.

PY - 2012

Y1 - 2012

N2 - Comparative analysis of the transport activity of proton pumps (plasmalemma H+ATPase, vacuolar H+ATPase, and vacuolar H+pyrophosphatase) in the membrane preparations obtained from coleoptile cells of etiolated maize seedlings (Zea mays L.) was carried out. The highest level of vacuolar pyrophosphatase activity was observed during the early development of coleoptile cells under growth intensification through the elongation. The role of ATPase pumps of tonoplast and plasmalemma in the transport of hydrogen ions increases during further development. The plasmalemma activity in this process is higher. When the growth stops, the activity of proton pumps becomes significantly lower. Nevertheless, their substrate specificity and sensitivity to proton pump inhibitors do not change, which can be an evidence of physiological significance of pumps in the maintenance of cell homeostasis.

AB - Comparative analysis of the transport activity of proton pumps (plasmalemma H+ATPase, vacuolar H+ATPase, and vacuolar H+pyrophosphatase) in the membrane preparations obtained from coleoptile cells of etiolated maize seedlings (Zea mays L.) was carried out. The highest level of vacuolar pyrophosphatase activity was observed during the early development of coleoptile cells under growth intensification through the elongation. The role of ATPase pumps of tonoplast and plasmalemma in the transport of hydrogen ions increases during further development. The plasmalemma activity in this process is higher. When the growth stops, the activity of proton pumps becomes significantly lower. Nevertheless, their substrate specificity and sensitivity to proton pump inhibitors do not change, which can be an evidence of physiological significance of pumps in the maintenance of cell homeostasis.

KW - Zea mays

KW - coleoptile

KW - P-type and V-type H+-ATPases

KW - V-type H+pyrophosphatase

KW - proton transport

KW - vesicles

U2 - 10.1134/S1062360412060070

DO - 10.1134/S1062360412060070

M3 - Article

VL - 43

SP - 342

EP - 352

JO - Russian Journal of Developmental Biology

JF - Russian Journal of Developmental Biology

SN - 1062-3604

IS - 6

ER -