TY - JOUR

T1 - Agricultural Crops Grown in Laboratory Conditions on Chernevaya Taiga Soil Demonstrate Unique Composition of the Rhizosphere Microbiota

AU - Kravchenko , Irina

AU - Rayko , Mikhail

AU - Tikhonova, Ekaterina

AU - Konopkin, Aleksey

AU - Evgeny Abakumov , Evgeny Abakumov

AU - Lapidus , Alla

N1 - Kravchenko, I.; Rayko, M.; Tikhonova, E.; Konopkin, A.; Abakumov, E.; Lapidus, A. Agricultural Crops Grown in Laboratory Conditions on Chernevaya Taiga Soil Demonstrate Unique Composition of the Rhizosphere Microbiota. Microorganisms 2022, 10, 2171. https://doi.org/10.3390/microorganisms10112171

PY - 2022/10/31

Y1 - 2022/10/31

N2 - Chernevaya taiga in West Siberia is a unique environment, with gigantism of grasses and shrubs. Exceptionally high productivity of plants is determined by the synergistic interaction of various factors, with a special role belonging to microorganisms colonizing the plant roots. This research explored whether agricultural plants can recruit specific microorganisms from within virgin Chernevaya Umbrisol and thus increase their productivity. Radish and wheat plants were grown on the Umbrisol (T1) and control Retisol of Scotch pine forest stand (T3) soils in the phytotron, and then a bacterial community analysis of the rhizosphere was performed using high-throughput sequencing of the 16S rRNA genes. In laboratory experiments, the plant physiological parameters were significantly higher when growing on the Umbrisol as compared to the Retisol. Bacterial diversity in T1 soil was considerably higher than in the control sample, and the principal coordinate analysis demonstrated apparent differences in the bacterial communities associated with the plants. Agricultural plants growing in the T1 soil form specific prokaryotic communities, with dominant genera Chthoniobacter, Pseudomonas, Burkholderia, and Massilia. These communities also include less abundant but essential for plant growth nitrifiers Cand. Nitrosocosmius and Nitrospira, and representatives of Proteobacteria, Bacilli, and Actinobacteria, known to be gibberellin-producers.

AB - Chernevaya taiga in West Siberia is a unique environment, with gigantism of grasses and shrubs. Exceptionally high productivity of plants is determined by the synergistic interaction of various factors, with a special role belonging to microorganisms colonizing the plant roots. This research explored whether agricultural plants can recruit specific microorganisms from within virgin Chernevaya Umbrisol and thus increase their productivity. Radish and wheat plants were grown on the Umbrisol (T1) and control Retisol of Scotch pine forest stand (T3) soils in the phytotron, and then a bacterial community analysis of the rhizosphere was performed using high-throughput sequencing of the 16S rRNA genes. In laboratory experiments, the plant physiological parameters were significantly higher when growing on the Umbrisol as compared to the Retisol. Bacterial diversity in T1 soil was considerably higher than in the control sample, and the principal coordinate analysis demonstrated apparent differences in the bacterial communities associated with the plants. Agricultural plants growing in the T1 soil form specific prokaryotic communities, with dominant genera Chthoniobacter, Pseudomonas, Burkholderia, and Massilia. These communities also include less abundant but essential for plant growth nitrifiers Cand. Nitrosocosmius and Nitrospira, and representatives of Proteobacteria, Bacilli, and Actinobacteria, known to be gibberellin-producers.

KW - metagenomics

KW - plant growth promoting rhizobacteria (PGPR)

KW - plant gigantism

KW - Umbrisol

KW - Retisol

KW - Chernevaya taiga

KW - metagenomics

KW - rhizosphere microbiome

KW - Chernevaya taiga

KW - Umbrisol

KW - Retisol

KW - plant gigantism

KW - lant growth promoting rhizobacteria (PGPR)

UR - https://www.mendeley.com/catalogue/823b23fa-dfc0-337c-8c99-de89602e32c0/

U2 - 10.3390/microorganisms10112171

DO - 10.3390/microorganisms10112171

M3 - Article

VL - 10

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 11

M1 - 2171

ER -