TY - JOUR

T1 - Adduct of fullerene C60 with l-arginine: interaction with DNA, genotoxic and mutagenic properties

AU - Kukaliia, Olegi N.

AU - Агеев, Сергей Вадимович

AU - Петров, Андрей Витальевич

AU - Шемчук, Ольга Сергеевна

AU - Самбук, Елена Викторовна

AU - Румянцев, Андрей Михайлович

AU - Дададжанов, Д. Р.

AU - Korzhenevskaiaa , Marina

AU - Молчанов, Олег Евгеньевич

AU - Майстренко, Дмитрий Николаевич

AU - Мурин, Игорь Васильевич

AU - Шаройко, Владимир Владимирович

AU - Семёнов, Константин Николаевич

PY - 2024/5/6

Y1 - 2024/5/6

N2 - The paper presents a physicochemical study of the water-soluble adduct of C60 with l-arginine, including a quantum chemical determination of the stability of isomers, measurement of antiradical activity towards the ABTS radical, determination of the lipophilicity coefficient, particle size distribution in aqueous solutions and calculation of ζ-potentials. A separate part of the article is devoted to the study of the effect of the C60 adduct with l-arginine on DNA in in vitro experiments (determination of the binding constant using isothermal titration calorimetry, study of genotoxicity, DNA melting temperature, effect on DNA circular dichroism spectra). Data are also presented on the biodistribution of the C60 adduct with l-arginine in the organs and tissues of animals and its effect on clinical and biochemical blood parameters. The mutagenic effect of the C60 adduct with l-arginine was determined in vivo on a model of mice abnormal sperm heads. It was shown that the adduct is in a neutral state in physiological solution, has an affinity to both aqueous and lipid phases (logPow = 0.23), and forms a complex with DNA (Ka = 4.59 106 M −1), while it does not have cyto- and genotoxicity in vitro and mutagenicity in vivo.

AB - The paper presents a physicochemical study of the water-soluble adduct of C60 with l-arginine, including a quantum chemical determination of the stability of isomers, measurement of antiradical activity towards the ABTS radical, determination of the lipophilicity coefficient, particle size distribution in aqueous solutions and calculation of ζ-potentials. A separate part of the article is devoted to the study of the effect of the C60 adduct with l-arginine on DNA in in vitro experiments (determination of the binding constant using isothermal titration calorimetry, study of genotoxicity, DNA melting temperature, effect on DNA circular dichroism spectra). Data are also presented on the biodistribution of the C60 adduct with l-arginine in the organs and tissues of animals and its effect on clinical and biochemical blood parameters. The mutagenic effect of the C60 adduct with l-arginine was determined in vivo on a model of mice abnormal sperm heads. It was shown that the adduct is in a neutral state in physiological solution, has an affinity to both aqueous and lipid phases (logPow = 0.23), and forms a complex with DNA (Ka = 4.59 106 M −1), while it does not have cyto- and genotoxicity in vitro and mutagenicity in vivo.

KW - Fullerene

KW - biodistribution

KW - cytotoxicity

KW - genotoxicity

KW - mutagenicity

UR - https://www.mendeley.com/catalogue/9971cac3-8143-3620-88f7-8f2de779cdd2/

U2 - 10.1080/1536383x.2024.2344768

DO - 10.1080/1536383x.2024.2344768

M3 - Article

VL - 32

SP - 916

EP - 927

JO - Fullerenes Nanotubes and Carbon Nanostructures

JF - Fullerenes Nanotubes and Carbon Nanostructures

SN - 1536-383X

IS - 10

ER -