TY - JOUR

T1 - Activation of the spinal neuronal network responsible for visceral control during locomotion

AU - Merkulyeva, Natalia

AU - Lyakhovetskii, Vsevolod

AU - Veshchitskii, Aleksandr

AU - Bazhenova, Elena

AU - Gorskii, Oleg

AU - Musienko, Pavel

PY - 2019/10/1

Y1 - 2019/10/1

N2 - It has been established that stepping of the decerebrate cat was accompanied by involvement of the urinary system: external urethral sphincter (EUS) and detrusor muscle activation, as well as the corresponding increase of the intravesical pressure. Detrusor and EUS evoked EMG activity matched the limbs locomotor movements. Immunohistochemical labeling of the immediate early gene c-fos expression was used to reveal the neural mechanisms of such somatovisceral interconnection within the sacral neural pathways. Study showed that two locomotor modes (forward and backward walking) had significantly different kinematic features. Combining the different immunohistochemical methods, we found that many c-fos-immunopositive nuclei were localized within several visceral areas of the S2 spinal segment which matched the sacral parasympathetic nucleus and dorsal gray commissure. Cats stepping backward had 4-fold more c-fos-immunopositive nuclei within the ventrolateral part of the sacral parasympathetic nucleus apparently correspondent to the “lateral band” contained cells controlling bladder function. The present work provides the direct evidences of visceral neurons activation depending on the specific of locomotor pattern and confirms the somatovisceral integration carrying out on the spinal cord level.

AB - It has been established that stepping of the decerebrate cat was accompanied by involvement of the urinary system: external urethral sphincter (EUS) and detrusor muscle activation, as well as the corresponding increase of the intravesical pressure. Detrusor and EUS evoked EMG activity matched the limbs locomotor movements. Immunohistochemical labeling of the immediate early gene c-fos expression was used to reveal the neural mechanisms of such somatovisceral interconnection within the sacral neural pathways. Study showed that two locomotor modes (forward and backward walking) had significantly different kinematic features. Combining the different immunohistochemical methods, we found that many c-fos-immunopositive nuclei were localized within several visceral areas of the S2 spinal segment which matched the sacral parasympathetic nucleus and dorsal gray commissure. Cats stepping backward had 4-fold more c-fos-immunopositive nuclei within the ventrolateral part of the sacral parasympathetic nucleus apparently correspondent to the “lateral band” contained cells controlling bladder function. The present work provides the direct evidences of visceral neurons activation depending on the specific of locomotor pattern and confirms the somatovisceral integration carrying out on the spinal cord level.

KW - Bladder function

KW - C-fos

KW - Cat

KW - Electrical epidural stimulation

KW - Locomotor network

KW - Parasympathetic neurons

KW - Spinal cord

KW - LOCALIZATION

KW - DECEREBRATE

KW - PREGANGLIONIC NEURONS

KW - LOWER URINARY-TRACT

KW - EPIDURAL ELECTRICAL-STIMULATION

KW - URETHRAL SPHINCTER ACTIVITY

KW - CAT

KW - BLADDER

KW - CORD STIMULATION

KW - C-FOS EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=85068173711&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/activation-spinal-neuronal-network-responsible-visceral-control-during-locomotion

U2 - 10.1016/j.expneurol.2019.112986

DO - 10.1016/j.expneurol.2019.112986

M3 - Article

C2 - 31254518

AN - SCOPUS:85068173711

VL - 320

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

M1 - 112986

ER -