TY - JOUR

T1 - Actin cytoskeleton rearrangements during the gravitropic response of Arabidopsis roots

AU - Pozhvanov, G.A.

AU - Suslov, D.V.

AU - Medvedev, S.S.

PY - 2013

Y1 - 2013

N2 - Gravitropic response is a plant growth response against changing its position relative to the gravity vector. In the present work we studied actin cytoskeleton rearrangements during Arabidopsis root gravitropic response. Two alternative approaches were used to visualize actin microfilaments: histochemical staining of fixed roots with rhodamine-phalloidin and live imaging of microfilaments in GFP-fABD2 transgenic plants. The curvature of actin microfilaments was shown to be increased within 30–60 min of gravistimulation, the fraction of axially oriented microfilaments decreased with a concomitant increase in the fraction of oblique and transversally oriented microfilaments. Methodological issues of actin cytoskeleton visualization in the study of Arabidopsis root gravitropic response, as well as the role of microfilaments at the stages of gravity perception, signal transduction and gravitropic bending formation are discussed. It is concluded that the actin cytoskeleton rearrangements observed are associated wi

AB - Gravitropic response is a plant growth response against changing its position relative to the gravity vector. In the present work we studied actin cytoskeleton rearrangements during Arabidopsis root gravitropic response. Two alternative approaches were used to visualize actin microfilaments: histochemical staining of fixed roots with rhodamine-phalloidin and live imaging of microfilaments in GFP-fABD2 transgenic plants. The curvature of actin microfilaments was shown to be increased within 30–60 min of gravistimulation, the fraction of axially oriented microfilaments decreased with a concomitant increase in the fraction of oblique and transversally oriented microfilaments. Methodological issues of actin cytoskeleton visualization in the study of Arabidopsis root gravitropic response, as well as the role of microfilaments at the stages of gravity perception, signal transduction and gravitropic bending formation are discussed. It is concluded that the actin cytoskeleton rearrangements observed are associated wi

KW - Arabidopsis thaliana

KW - gravitropism

KW - cytoskeleton

KW - actin

KW - root

KW - extension growth

U2 - 10.1134/S1990519X13020120

DO - 10.1134/S1990519X13020120

M3 - Article

VL - 7

SP - 185

EP - 191

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 2

ER -