Standard

Accurate isoform discovery with IsoQuant using long reads. / Prjibelski, Andrey D; Lapidus, Alla L; Joglekar, Anoushka; Smetanin, Alexander; Mikheenko, Alla; Jarroux, Julien; Tilgner, Hagen U.

в: Nature Biotechnology, Том 41, № 7, 07.2023, стр. 915-918.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Prjibelski, AD, Lapidus, AL, Joglekar, A, Smetanin, A, Mikheenko, A, Jarroux, J & Tilgner, HU 2023, 'Accurate isoform discovery with IsoQuant using long reads', Nature Biotechnology, Том. 41, № 7, стр. 915-918. https://doi.org/10.1038/s41587-022-01565-y

APA

Prjibelski, A. D., Lapidus, A. L., Joglekar, A., Smetanin, A., Mikheenko, A., Jarroux, J., & Tilgner, H. U. (2023). Accurate isoform discovery with IsoQuant using long reads. Nature Biotechnology, 41(7), 915-918. https://doi.org/10.1038/s41587-022-01565-y

Vancouver

Prjibelski AD, Lapidus AL, Joglekar A, Smetanin A, Mikheenko A, Jarroux J и пр. Accurate isoform discovery with IsoQuant using long reads. Nature Biotechnology. 2023 Июль;41(7):915-918. https://doi.org/10.1038/s41587-022-01565-y

Author

Prjibelski, Andrey D ; Lapidus, Alla L ; Joglekar, Anoushka ; Smetanin, Alexander ; Mikheenko, Alla ; Jarroux, Julien ; Tilgner, Hagen U. / Accurate isoform discovery with IsoQuant using long reads. в: Nature Biotechnology. 2023 ; Том 41, № 7. стр. 915-918.

BibTeX

@article{1b627f64b74c4f1fba8dc47af98f0b3a,
title = "Accurate isoform discovery with IsoQuant using long reads",
abstract = "Annotating newly sequenced genomes and determining alternative isoforms from long-read RNA data are complex and incompletely solved problems. Here we present IsoQuant-a computational tool using intron graphs that accurately reconstructs transcripts both with and without reference genome annotation. For novel transcript discovery, IsoQuant reduces the false-positive rate fivefold and 2.5-fold for Oxford Nanopore reference-based or reference-free mode, respectively. IsoQuant also improves performance for Pacific Biosciences data.",
keywords = "Genome informatics, software, Sequence Analysis, RNA, RNA, Protein Isoforms/genetics, High-Throughput Nucleotide Sequencing, Genome, Sequence Analysis, DNA",
author = "Prjibelski, {Andrey D} and Lapidus, {Alla L} and Anoushka Joglekar and Alexander Smetanin and Alla Mikheenko and Julien Jarroux and Tilgner, {Hagen U}",
note = "Prjibelski, A.D., Mikheenko, A., Joglekar, A. et al. Accurate isoform discovery with IsoQuant using long reads. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-022-01565-y",
year = "2023",
month = jul,
doi = "10.1038/s41587-022-01565-y",
language = "English",
volume = "41",
pages = "915--918",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "7",

}

RIS

TY - JOUR

T1 - Accurate isoform discovery with IsoQuant using long reads

AU - Prjibelski, Andrey D

AU - Lapidus, Alla L

AU - Joglekar, Anoushka

AU - Smetanin, Alexander

AU - Mikheenko, Alla

AU - Jarroux, Julien

AU - Tilgner, Hagen U

N1 - Prjibelski, A.D., Mikheenko, A., Joglekar, A. et al. Accurate isoform discovery with IsoQuant using long reads. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-022-01565-y

PY - 2023/7

Y1 - 2023/7

N2 - Annotating newly sequenced genomes and determining alternative isoforms from long-read RNA data are complex and incompletely solved problems. Here we present IsoQuant-a computational tool using intron graphs that accurately reconstructs transcripts both with and without reference genome annotation. For novel transcript discovery, IsoQuant reduces the false-positive rate fivefold and 2.5-fold for Oxford Nanopore reference-based or reference-free mode, respectively. IsoQuant also improves performance for Pacific Biosciences data.

AB - Annotating newly sequenced genomes and determining alternative isoforms from long-read RNA data are complex and incompletely solved problems. Here we present IsoQuant-a computational tool using intron graphs that accurately reconstructs transcripts both with and without reference genome annotation. For novel transcript discovery, IsoQuant reduces the false-positive rate fivefold and 2.5-fold for Oxford Nanopore reference-based or reference-free mode, respectively. IsoQuant also improves performance for Pacific Biosciences data.

KW - Genome informatics

KW - software

KW - Sequence Analysis, RNA

KW - RNA

KW - Protein Isoforms/genetics

KW - High-Throughput Nucleotide Sequencing

KW - Genome

KW - Sequence Analysis, DNA

UR - https://www.mendeley.com/catalogue/d383cf4f-81f8-3571-954b-1ddce8f86e14/

U2 - 10.1038/s41587-022-01565-y

DO - 10.1038/s41587-022-01565-y

M3 - Article

C2 - 36593406

VL - 41

SP - 915

EP - 918

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 7

ER -

ID: 106999301