TY - JOUR

T1 - A novel uncultured heterotrophic bacterial associate of the cyanobacterium Moorea producens JHB

AU - Cummings, S.L.

AU - Barbé, D.

AU - Leao, T.F.

AU - Korobeynikov, Anton

AU - Engene, N.

AU - Glukhov, E.

AU - Gerwick, W.H.

AU - Gerwick, L.

PY - 2016

Y1 - 2016

N2 - © 2016 The Author(s).Background: Filamentous tropical marine cyanobacteria such as Moorea producens strain JHB possess a rich community of heterotrophic bacteria on their polysaccharide sheaths; however, these bacterial communities have not yet been adequately studied or characterized. Results and discussion: Through efforts to sequence the genome of this cyanobacterial strain, the 5.99 MB genome of an unknown bacterium emerged from the metagenomic information, named here as Mor1. Analysis of its genome revealed that the bacterium is heterotrophic and belongs to the phylum Acidobacteria, subgroup 22; however, it is only 85 % identical to the nearest cultured representative. Comparative genomics further revealed that Mor1 has a large number of genes involved in transcriptional regulation, is completely devoid of transposases, is not able to synthesize the full complement of proteogenic amino acids and appears to lack genes for nitrate uptake. Mor1 was found to be present in lab cultures of M. producens collect

AB - © 2016 The Author(s).Background: Filamentous tropical marine cyanobacteria such as Moorea producens strain JHB possess a rich community of heterotrophic bacteria on their polysaccharide sheaths; however, these bacterial communities have not yet been adequately studied or characterized. Results and discussion: Through efforts to sequence the genome of this cyanobacterial strain, the 5.99 MB genome of an unknown bacterium emerged from the metagenomic information, named here as Mor1. Analysis of its genome revealed that the bacterium is heterotrophic and belongs to the phylum Acidobacteria, subgroup 22; however, it is only 85 % identical to the nearest cultured representative. Comparative genomics further revealed that Mor1 has a large number of genes involved in transcriptional regulation, is completely devoid of transposases, is not able to synthesize the full complement of proteogenic amino acids and appears to lack genes for nitrate uptake. Mor1 was found to be present in lab cultures of M. producens collect

U2 - 10.1186/s12866-016-0817-1

DO - 10.1186/s12866-016-0817-1

M3 - Article

VL - 16

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

ER -