A new approach for analysis of polyprenols by a combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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A new approach for analysis of polyprenols by a combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. / Gladchuk, Alexey S.; Krasnov, Konstantin A.; Keltsieva, Olga A.; Kalninia, Yana K.; Alexandrova, Marina L.; Ivanov, Nikita S.; Muradymov, Marat Z.; Krasnov, Nikolai V.; Reynyuk, Vladimir L.; Sukhodolov, Nikolai G.; Podolskaya, Ekaterina P.

в: Rapid Communications in Mass Spectrometry, Том 35, № 21, e9185, 15.11.2021.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Author

Gladchuk, Alexey S. ; Krasnov, Konstantin A. ; Keltsieva, Olga A. ; Kalninia, Yana K. ; Alexandrova, Marina L. ; Ivanov, Nikita S. ; Muradymov, Marat Z. ; Krasnov, Nikolai V. ; Reynyuk, Vladimir L. ; Sukhodolov, Nikolai G. ; Podolskaya, Ekaterina P. / A new approach for analysis of polyprenols by a combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. в: Rapid Communications in Mass Spectrometry. 2021 ; Том 35, № 21.

BibTeX

@article{83f746d9aee24ad1b83bb7f5ce206445,

title = "A new approach for analysis of polyprenols by a combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry",

abstract = "Rationale: The polyprenols are involved in some essential biosynthetic pathways and serve as ubiquitous components of cellular membranes, so their fingerprinting in natural samples is of great interest. Previous studies indicate that due to the high hydrophobicity of polyprenols their direct analysis by mass spectrometry with soft ionization techniques may be difficult and require preliminary off-line derivatization. Hence, a method for rapid and sensitive screening of polyprenols is required. Methods: A combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for analysis of the polyprenol profile of Abies sibirica L. extract. Polyprenol-based monolayers were formed at the interphase of aqueous barium acetate solution, supplemented with 2,5-dihydroxybenzoic acid, and an n-hexane solution of polyprenols directly on a MALDI target plate. Results: Peaks corresponding to [M − H + Ba]+ ions were observed in the MALDI-TOF mass spectra of polyprenols. A total of nine polyprenol homologues were identified with a polyprenol of 16 isoprene units dominating. The limit of detection was established at the level of 6 pg. Possible mechanisms of formation of [M − H + Ba]+ ions of polyprenols were discussed. Conclusions: The proposed approach can be suitable for high-throughput screening of polyprenols in biological samples of different origin due to easy sample preparation and high sensitivity.",

keywords = "LONG-CHAIN POLYPRENOLS, FAST-ATOM-BOMBARDMENT, POLYISOPRENOID ALCOHOLS, CHROMATOGRAPHY, LEAVES, METABOLITES",

author = "Gladchuk, {Alexey S.} and Krasnov, {Konstantin A.} and Keltsieva, {Olga A.} and Kalninia, {Yana K.} and Alexandrova, {Marina L.} and Ivanov, {Nikita S.} and Muradymov, {Marat Z.} and Krasnov, {Nikolai V.} and Reynyuk, {Vladimir L.} and Sukhodolov, {Nikolai G.} and Podolskaya, {Ekaterina P.}",

note = "Publisher Copyright: {\textcopyright} 2021 John Wiley & Sons Ltd",

year = "2021",

month = nov,

day = "15",

doi = "10.1002/rcm.9185",

language = "English",

volume = "35",

journal = "Rapid Communications in Mass Spectrometry",

issn = "0951-4198",

publisher = "Wiley-Blackwell",

number = "21",

}

RIS

TY - JOUR

T1 - A new approach for analysis of polyprenols by a combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

AU - Gladchuk, Alexey S.

AU - Krasnov, Konstantin A.

AU - Keltsieva, Olga A.

AU - Kalninia, Yana K.

AU - Alexandrova, Marina L.

AU - Ivanov, Nikita S.

AU - Muradymov, Marat Z.

AU - Krasnov, Nikolai V.

AU - Reynyuk, Vladimir L.

AU - Sukhodolov, Nikolai G.

AU - Podolskaya, Ekaterina P.

PY - 2021/11/15

Y1 - 2021/11/15

N2 - Rationale: The polyprenols are involved in some essential biosynthetic pathways and serve as ubiquitous components of cellular membranes, so their fingerprinting in natural samples is of great interest. Previous studies indicate that due to the high hydrophobicity of polyprenols their direct analysis by mass spectrometry with soft ionization techniques may be difficult and require preliminary off-line derivatization. Hence, a method for rapid and sensitive screening of polyprenols is required. Methods: A combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for analysis of the polyprenol profile of Abies sibirica L. extract. Polyprenol-based monolayers were formed at the interphase of aqueous barium acetate solution, supplemented with 2,5-dihydroxybenzoic acid, and an n-hexane solution of polyprenols directly on a MALDI target plate. Results: Peaks corresponding to [M − H + Ba]+ ions were observed in the MALDI-TOF mass spectra of polyprenols. A total of nine polyprenol homologues were identified with a polyprenol of 16 isoprene units dominating. The limit of detection was established at the level of 6 pg. Possible mechanisms of formation of [M − H + Ba]+ ions of polyprenols were discussed. Conclusions: The proposed approach can be suitable for high-throughput screening of polyprenols in biological samples of different origin due to easy sample preparation and high sensitivity.

AB - Rationale: The polyprenols are involved in some essential biosynthetic pathways and serve as ubiquitous components of cellular membranes, so their fingerprinting in natural samples is of great interest. Previous studies indicate that due to the high hydrophobicity of polyprenols their direct analysis by mass spectrometry with soft ionization techniques may be difficult and require preliminary off-line derivatization. Hence, a method for rapid and sensitive screening of polyprenols is required. Methods: A combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for analysis of the polyprenol profile of Abies sibirica L. extract. Polyprenol-based monolayers were formed at the interphase of aqueous barium acetate solution, supplemented with 2,5-dihydroxybenzoic acid, and an n-hexane solution of polyprenols directly on a MALDI target plate. Results: Peaks corresponding to [M − H + Ba]+ ions were observed in the MALDI-TOF mass spectra of polyprenols. A total of nine polyprenol homologues were identified with a polyprenol of 16 isoprene units dominating. The limit of detection was established at the level of 6 pg. Possible mechanisms of formation of [M − H + Ba]+ ions of polyprenols were discussed. Conclusions: The proposed approach can be suitable for high-throughput screening of polyprenols in biological samples of different origin due to easy sample preparation and high sensitivity.

KW - LONG-CHAIN POLYPRENOLS

KW - FAST-ATOM-BOMBARDMENT

KW - POLYISOPRENOID ALCOHOLS

KW - CHROMATOGRAPHY

KW - LEAVES

KW - METABOLITES

UR - http://www.scopus.com/inward/record.url?scp=85116328702&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d2fd9763-f43e-3375-8862-b4cf1ab2cbe3/

U2 - 10.1002/rcm.9185

DO - 10.1002/rcm.9185

M3 - Article

C2 - 34460139

AN - SCOPUS:85116328702

VL - 35

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 21

M1 - e9185

ER -

ID: 88336699