TY - JOUR

T1 - АКТИВАЦИЯ ЭКСПРЕССИИ ТРАНСКРИПЦИОННОГО ФАКТОРА ZBTB16 ПРИ ОСТЕОГЕННОЙ ДИФФЕРЕНЦИРОВКЕ СТВОЛОВЫХ КЛЕТОК МЕЗЕНХИМНОГО РЯДА

AU - Malashicheva, A. B.

N1 - Publisher Copyright: © 2021 NII KPSSZ. All Rights Reserved.

PY - 2021/9/25

Y1 - 2021/9/25

N2 - Conclusion The study of modulation of cellular signals by ZBTB16, when activating or suppressing the work of a transcriptional factor, in the future may bring us closer to the ability to enhance the regenerative abilities of bone tissue cells or, conversely, prevent calcification of the aortic valve tissues.Aim Calcified aortic valve stenosis is the third leading cause of cardiovascular disease. The mechanisms underlying this process remain unclear, however, it is known that they are largely similar to the formation of bone tissue during embryonic development, as well as in the postnatal period during regeneration. There is evidence for the involvement of Zinc Finger and BTB Domain Containing 16 (ZBTB16) in skeletal development. At the same time, a number of studies carried out on different types of cell cultures indicate a contradictory and ambiguous effect of ZBTB16 on RUNX2 expression. Thus, the aim of this study was to investigate the dynamic variability of ZBTB16 expression, as well as its role in aortic valve calcification. Methods The study used different types of mesenchymal cells cultures - aortic valve interstitial cells, umbilical cord mesenchymal stem cells, ligament stem cells and dental pulp stem cells. Changes in ZBTB16 and RUNX2 expression levels under the influence of osteogenic stimuli, as well as during exogenous activation of ZBTB16, were analyzed using real-time PCR. Expression levels of some osteogenic markers - BMP2,4, COL1A1, IBSP, DLX2, PDK4 - were analyzed in the interstitial cells of the aortic valve. Results The results of the study indicate that a significant increase in the expression of ZBTB16 is observed during the induction of osteogenic differentiation of various cell cultures - interstitial cells of the aortic valve, mesenchymal stem cells of the umbilical cord, stem cells of the ligaments and dental pulp. Apparently, the processes of osteogenic differentiation of aortic valve interstitial cells, in the presence of dexamethasone in cultivation medium, are provided through RUNX2-dependent signaling for the further activation of osteogenic markers.

AB - Conclusion The study of modulation of cellular signals by ZBTB16, when activating or suppressing the work of a transcriptional factor, in the future may bring us closer to the ability to enhance the regenerative abilities of bone tissue cells or, conversely, prevent calcification of the aortic valve tissues.Aim Calcified aortic valve stenosis is the third leading cause of cardiovascular disease. The mechanisms underlying this process remain unclear, however, it is known that they are largely similar to the formation of bone tissue during embryonic development, as well as in the postnatal period during regeneration. There is evidence for the involvement of Zinc Finger and BTB Domain Containing 16 (ZBTB16) in skeletal development. At the same time, a number of studies carried out on different types of cell cultures indicate a contradictory and ambiguous effect of ZBTB16 on RUNX2 expression. Thus, the aim of this study was to investigate the dynamic variability of ZBTB16 expression, as well as its role in aortic valve calcification. Methods The study used different types of mesenchymal cells cultures - aortic valve interstitial cells, umbilical cord mesenchymal stem cells, ligament stem cells and dental pulp stem cells. Changes in ZBTB16 and RUNX2 expression levels under the influence of osteogenic stimuli, as well as during exogenous activation of ZBTB16, were analyzed using real-time PCR. Expression levels of some osteogenic markers - BMP2,4, COL1A1, IBSP, DLX2, PDK4 - were analyzed in the interstitial cells of the aortic valve. Results The results of the study indicate that a significant increase in the expression of ZBTB16 is observed during the induction of osteogenic differentiation of various cell cultures - interstitial cells of the aortic valve, mesenchymal stem cells of the umbilical cord, stem cells of the ligaments and dental pulp. Apparently, the processes of osteogenic differentiation of aortic valve interstitial cells, in the presence of dexamethasone in cultivation medium, are provided through RUNX2-dependent signaling for the further activation of osteogenic markers.

KW - Aortic valve interstitial cells

KW - Mesenchymal stem cells

KW - Osteogenic differentiation

KW - RUNX2

KW - ZBTB16

KW - Aortic valve interstitial cells

KW - Mesenchymal stem cells

KW - Osteogenic differentiation

KW - RUNX2

KW - ZBTB16

UR - http://www.scopus.com/inward/record.url?scp=85117016726&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/ced39ca7-ad50-3be0-ad4c-7c9090e40924/

U2 - 10.17802/2306-1278-2021-10-3-44-55

DO - 10.17802/2306-1278-2021-10-3-44-55

M3 - статья

AN - SCOPUS:85117016726

VL - 10

SP - 44

EP - 55

JO - Комплексные проблемы сердечно-сосудистых заболеваний

JF - Комплексные проблемы сердечно-сосудистых заболеваний

SN - 2306-1278

IS - 3

ER -