TY - JOUR

T1 - ТРИЦИКЛИЧЕСКИЙ АНТИДЕПРЕССАНТ АМИТРИПТИЛИН ПОДАВЛЯЕТ Са2+-ОТВЕТЫ В ПЕРИТОНЕАЛЬНЫХ МАКРОФАГАХ КРЫСЫ

AU - Миленина, Лидия Сергеевна

AU - Крутецкая, Зоя Иринарховна

AU - Антонов, Виктор Георгиевич

AU - Крутецкая, Нина Иринарховна

N1 - Миленина Л.С., Крутецкая З.И., Антонов В.Г., Крутецкая Н.И. ТРИЦИКЛИЧЕСКИЙ АНТИДЕПРЕССАНТ АМИТРИПТИЛИН ПОДАВЛЯЕТ Са2+-ОТВЕТЫ В ПЕРИТОНЕАЛЬНЫХ МАКРОФАГАХ КРЫСЫ. ЦИТОЛОГИЯ. 2024. Т. 66. № 2. С. 161-172.

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Amitriptyline is a tricyclic antidepressant widely used in clinical practice for the treatment of anxiety, depression and chronic pain. These drugs have a multifaceted effect on cellular processes. One of their targets is sigma-1 receptors. Sigma-1 receptors are molecular chaperones located in endoplasmic reticulum membrane; they are characterized by a unique structure and pharmacological profile. Sigma-1 receptors regulate many cellular processes in health and disease, including Ca2+ signaling. Using Fura-2AM microfluorimetry, it was shown for the first time that sigma-1 receptor agonist, antidepressant amitriptyline, significantly suppresses both Ca2+ mobilization from intracellular Ca2+-stores and subsequent store-dependent Ca2+ entry into cells, induced by endoplasmic Ca2+-ATPase inhibitors thapsigargin and cyclopiazonic acid, as well as disulfide-containing immunomodulators glutoxim and molixan, in rat peritoneal macrophages. The results suggest the involvement of sigma-1 receptors in a complex signaling cascade induced by glutoxim or molixan, leading to an increase of intracellular Ca2+ concentration in macrophages. The results also indicate the participation of sigma-1 receptors in the regulation of store-dependent Ca2+ entry in macrophages.

AB - Amitriptyline is a tricyclic antidepressant widely used in clinical practice for the treatment of anxiety, depression and chronic pain. These drugs have a multifaceted effect on cellular processes. One of their targets is sigma-1 receptors. Sigma-1 receptors are molecular chaperones located in endoplasmic reticulum membrane; they are characterized by a unique structure and pharmacological profile. Sigma-1 receptors regulate many cellular processes in health and disease, including Ca2+ signaling. Using Fura-2AM microfluorimetry, it was shown for the first time that sigma-1 receptor agonist, antidepressant amitriptyline, significantly suppresses both Ca2+ mobilization from intracellular Ca2+-stores and subsequent store-dependent Ca2+ entry into cells, induced by endoplasmic Ca2+-ATPase inhibitors thapsigargin and cyclopiazonic acid, as well as disulfide-containing immunomodulators glutoxim and molixan, in rat peritoneal macrophages. The results suggest the involvement of sigma-1 receptors in a complex signaling cascade induced by glutoxim or molixan, leading to an increase of intracellular Ca2+ concentration in macrophages. The results also indicate the participation of sigma-1 receptors in the regulation of store-dependent Ca2+ entry in macrophages.

UR - https://www.mendeley.com/catalogue/5a257400-d48e-3098-89af-ceec16368408/

U2 - 10.31857/S0041377124020069

DO - 10.31857/S0041377124020069

M3 - статья

VL - 66

SP - 161

EP - 172

JO - Цитология

JF - Цитология

SN - 0041-3771

IS - 2

ER -