Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Разделение энантиомеров тирозина, триптофана и β-блокаторов методом капиллярного электрофореза с участием аминокислотной ионной жидкости 1-бутил-3-метилимидазолий L-пролинат [C4MIm][L-Pro] в качстве хирального селектора. / Kolobova, E. A.; Kartsova, L. A.; Alopina, E. V.; Smirnova, N. A.
в: АНАЛИТИКА И КОНТРОЛЬ, Том 22, № 1, 2018, стр. 51-60.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Разделение энантиомеров тирозина, триптофана и β-блокаторов методом капиллярного электрофореза с участием аминокислотной ионной жидкости 1-бутил-3-метилимидазолий L-пролинат [C4MIm][L-Pro] в качстве хирального селектора
AU - Kolobova, E. A.
AU - Kartsova, L. A.
AU - Alopina, E. V.
AU - Smirnova, N. A.
PY - 2018
Y1 - 2018
N2 - The current study is devoted to the synthesis of amino acid ionic 1-butyl-3-methylimidazolium L-prolinate [C4MIm][L-Pro] liquid and the investigation of its possibilities as a chiral selector for the separation of amino acids (tryptophan and tyrosine) and β-blockers (carvedilol and propranolol) enantiomers. The following factors affecting the resolution of amino acids stereoisomers under ligand exchange capillary electrophoresis were established: background electrolyte pH, concentration of chelate complex, molar ratio of metal and ligand and its nature. The enantioselectivity values of 1.25 for tryptophan enantiomers and 1.17 for tyrosine enantiomers were observed when the background electrolyte consisted of 50 mM borate buffer (pH = 12.2), 20 mM [C4MIm][L-Pro], and 10 mM CuSO4. The synergetic effect of the synthesized ionic liquid and (2-hydroxypropyl)-β-cyclodextrine when added to the background electrolyte content (20 mM NaH2PO4, pH = 2.5) was discovered during the separation of carvedilol and propranolol enantiomers. The achieved resolution for carvedilol (Rs = 1.1) and propranolol (Rs = 1.6) enantiomers allowed determining the enantiomers ratio of active ingredients in drug formulations.
AB - The current study is devoted to the synthesis of amino acid ionic 1-butyl-3-methylimidazolium L-prolinate [C4MIm][L-Pro] liquid and the investigation of its possibilities as a chiral selector for the separation of amino acids (tryptophan and tyrosine) and β-blockers (carvedilol and propranolol) enantiomers. The following factors affecting the resolution of amino acids stereoisomers under ligand exchange capillary electrophoresis were established: background electrolyte pH, concentration of chelate complex, molar ratio of metal and ligand and its nature. The enantioselectivity values of 1.25 for tryptophan enantiomers and 1.17 for tyrosine enantiomers were observed when the background electrolyte consisted of 50 mM borate buffer (pH = 12.2), 20 mM [C4MIm][L-Pro], and 10 mM CuSO4. The synergetic effect of the synthesized ionic liquid and (2-hydroxypropyl)-β-cyclodextrine when added to the background electrolyte content (20 mM NaH2PO4, pH = 2.5) was discovered during the separation of carvedilol and propranolol enantiomers. The achieved resolution for carvedilol (Rs = 1.1) and propranolol (Rs = 1.6) enantiomers allowed determining the enantiomers ratio of active ingredients in drug formulations.
KW - Amino acid ionic liquids
KW - Amino acids
KW - Chiral selector
KW - Enantioseparation
KW - Ligand exchange capillary electrophoresis
KW - Synergetic effect
KW - β-blockers
UR - http://www.scopus.com/inward/record.url?scp=85044416982&partnerID=8YFLogxK
U2 - 10.15826/analitika.2018.22.1.004
DO - 10.15826/analitika.2018.22.1.004
M3 - статья
AN - SCOPUS:85044416982
VL - 22
SP - 51
EP - 60
JO - АНАЛИТИКА И КОНТРОЛЬ
JF - АНАЛИТИКА И КОНТРОЛЬ
SN - 2073-1442
IS - 1
ER -
ID: 40041560