Сравнительный анализ экспрессии генов, связанных со стрессом, у двух линий гороха, контрастных по признаку устойчивости к кадмию

Standard

Сравнительный анализ экспрессии генов, связанных со стрессом, у двух линий гороха, контрастных по признаку устойчивости к кадмию. / Kulaeva, O. A.; Gribchenko, E. S.; Zorin, E. A.; Kliukova, M. S.; Zhukov, V. A.

в: Ecological Genetics, Том 16, № 4, 2018, стр. 75-84.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

BibTeX

@article{8768fdc99e9c499a95522878c56963d6,

title = "Сравнительный анализ экспрессии генов, связанных со стрессом, у двух линий гороха, контрастных по признаку устойчивости к кадмию",

abstract = "` Background. A major problem of the environmental pollution with heavy metals, including cadmium, requires an intensive study of the molecular and genetic mechanisms underlying the tolerance of plants to these toxic substances. In this study we present a comparative analysis of the expression of stress-related genes in two pea genotypes contrasting in tolerance to cadmium. Materials and methods. A unique mutant of pea SGECdt, characterized by the increased tolerance to cadmium, and initial line SGE were used. Gene expression was analyzed by Real Time PCR. results. In the line SGE cadmium increase the expression of genes, encoding catalase, chitinase, chitinase-like protein PRP4A and dirigent protein PI206. In the mutant SGECdt cadmium increase the expression of genes, encoding chitinase, glutathione reductase and defensin DRR230. In control samples expression of genes encoding PRP4A and DRRR230 was enhanced in mutant SGECdt versus line SGE. Conclusion. It was shown that, the reaction of the mutant SGECdt at the molecular level differs from that of the line SGE. In the mutant SGECdt, a change in the expression of a number of genes is observed, which may indicate that cadmium entering the cell causes activation of defense reactions.",

keywords = "Cadmium, Gene expression, Pea, Stress",

author = "Kulaeva, {O. A.} and Gribchenko, {E. S.} and Zorin, {E. A.} and Kliukova, {M. S.} and Zhukov, {V. A.}",

year = "2018",

doi = "10.17816/ecogen16475-84",

language = "русский",

volume = "16",

pages = "75--84",

journal = "ЭКОЛОГИЧЕСКАЯ ГЕНЕТИКА",

issn = "1811-0932",

publisher = "Эко-Вектор",

number = "4",

}

RIS

TY - JOUR

T1 - Сравнительный анализ экспрессии генов, связанных со стрессом, у двух линий гороха, контрастных по признаку устойчивости к кадмию

AU - Kulaeva, O. A.

AU - Gribchenko, E. S.

AU - Zorin, E. A.

AU - Kliukova, M. S.

AU - Zhukov, V. A.

PY - 2018

Y1 - 2018

N2 - ` Background. A major problem of the environmental pollution with heavy metals, including cadmium, requires an intensive study of the molecular and genetic mechanisms underlying the tolerance of plants to these toxic substances. In this study we present a comparative analysis of the expression of stress-related genes in two pea genotypes contrasting in tolerance to cadmium. Materials and methods. A unique mutant of pea SGECdt, characterized by the increased tolerance to cadmium, and initial line SGE were used. Gene expression was analyzed by Real Time PCR. results. In the line SGE cadmium increase the expression of genes, encoding catalase, chitinase, chitinase-like protein PRP4A and dirigent protein PI206. In the mutant SGECdt cadmium increase the expression of genes, encoding chitinase, glutathione reductase and defensin DRR230. In control samples expression of genes encoding PRP4A and DRRR230 was enhanced in mutant SGECdt versus line SGE. Conclusion. It was shown that, the reaction of the mutant SGECdt at the molecular level differs from that of the line SGE. In the mutant SGECdt, a change in the expression of a number of genes is observed, which may indicate that cadmium entering the cell causes activation of defense reactions.

AB - ` Background. A major problem of the environmental pollution with heavy metals, including cadmium, requires an intensive study of the molecular and genetic mechanisms underlying the tolerance of plants to these toxic substances. In this study we present a comparative analysis of the expression of stress-related genes in two pea genotypes contrasting in tolerance to cadmium. Materials and methods. A unique mutant of pea SGECdt, characterized by the increased tolerance to cadmium, and initial line SGE were used. Gene expression was analyzed by Real Time PCR. results. In the line SGE cadmium increase the expression of genes, encoding catalase, chitinase, chitinase-like protein PRP4A and dirigent protein PI206. In the mutant SGECdt cadmium increase the expression of genes, encoding chitinase, glutathione reductase and defensin DRR230. In control samples expression of genes encoding PRP4A and DRRR230 was enhanced in mutant SGECdt versus line SGE. Conclusion. It was shown that, the reaction of the mutant SGECdt at the molecular level differs from that of the line SGE. In the mutant SGECdt, a change in the expression of a number of genes is observed, which may indicate that cadmium entering the cell causes activation of defense reactions.

KW - Cadmium

KW - Gene expression

KW - Pea

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=85061407167&partnerID=8YFLogxK

U2 - 10.17816/ecogen16475-84

DO - 10.17816/ecogen16475-84

M3 - статья

AN - SCOPUS:85061407167

VL - 16

SP - 75

EP - 84

JO - ЭКОЛОГИЧЕСКАЯ ГЕНЕТИКА

JF - ЭКОЛОГИЧЕСКАЯ ГЕНЕТИКА

SN - 1811-0932

IS - 4

ER -

ID: 89279430