TY - JOUR

T1 - Исследование конформационных изменений молекулы ДНК, вызванных гамма-облучением в водно-этанольных растворах

AU - Paston, S V

AU - Zamotin, V V

AU - Zyrianova, I M

PY - 2003

Y1 - 2003

N2 - The intrinsic viscosity, optical anisotropy and spectral properties of DNA molecule gamma-irradiated with the doses of 10, 20 and 30 Gy in water-ethanol solutions with ethanol concentrations 0-6 mol/l are investigated in the work. Specific volume of DNA at all doses used shows a complex non-monotone dependence on the ethanol content with a peculiarity at the alcohol concentration corresponding to the destruction of water structure in the mixed solvent (so-called, critical concentration, 3.5 mol/l). Ethanol presence at the concentrations below the critical one protects macromolecule from the radiation action. At the alcohol concentrations larger the critical an inversion of the dose dependence of the DNA specific volume is observed. At that the equilibrium rigidity and secondary structure of macromolecule do not change noticeably. The results obtained indicate a significant role of the solvent structure in radiation damage of DNA molecule.

AB - The intrinsic viscosity, optical anisotropy and spectral properties of DNA molecule gamma-irradiated with the doses of 10, 20 and 30 Gy in water-ethanol solutions with ethanol concentrations 0-6 mol/l are investigated in the work. Specific volume of DNA at all doses used shows a complex non-monotone dependence on the ethanol content with a peculiarity at the alcohol concentration corresponding to the destruction of water structure in the mixed solvent (so-called, critical concentration, 3.5 mol/l). Ethanol presence at the concentrations below the critical one protects macromolecule from the radiation action. At the alcohol concentrations larger the critical an inversion of the dose dependence of the DNA specific volume is observed. At that the equilibrium rigidity and secondary structure of macromolecule do not change noticeably. The results obtained indicate a significant role of the solvent structure in radiation damage of DNA molecule.

KW - Animals

KW - Cattle

KW - Cesium Radioisotopes/pharmacology

KW - DNA/radiation effects

KW - DNA Damage

KW - Ethanol

KW - Gamma Rays

KW - Macromolecular Substances

KW - Models, Theoretical

KW - Nucleic Acid Conformation/radiation effects

KW - Radiation Dosage

KW - Solutions

KW - Spectrophotometry

KW - Thymus Gland/metabolism

KW - Viscosity

KW - Water

UR - https://pubmed.ncbi.nlm.nih.gov/14963929/

M3 - статья

C2 - 14963929

VL - 43

SP - 633

EP - 639

JO - РАДИАЦИОННАЯ БИОЛОГИЯ. РАДИОЭКОЛОГИЯ

JF - РАДИАЦИОННАЯ БИОЛОГИЯ. РАДИОЭКОЛОГИЯ

SN - 0869-8031

IS - 6

ER -