Background. Minimally invasive partial nephrectomy is the gold standard in the treatment of stage I malignant tumors. To date, there are a large number of techniques for performing partial nephrectomy. The desire to develop a technique that included all the positive characteristics and had no restrictions on use led to the creation of a normotonic zero ischaemia partial nephrectomy. Materials and methods. A retrospective analysis of 45 patients was performed. 1^st group included 24 (53.3 %) patients after laparoscopic normotonic zero ischaemia partial nephrectomy. 2^nd group included 21 (46.7 %) patients who underwent laparoscopic hypotonic zero ischaemia partial nephrectomy. All patients evaluated such surgical parameters as the surgery time, the blood loss, and the duration of hospitalization. To assess pre-operative renal function, the CKD-EPI equation was used to calculate estimate glomerular filtration rate. Results. All patients were demographically comparable. Patients were also evenly distributed in terms of resection complexity according to the RENAL nephrometric scale. Acute kidney injury rate was significantly higher in the hypotension group: relative risk 5.4 (95 % confidence interval 1.59–20.55), odds ratio 11.3 (95 % confidence interval 2.04–59.2); p = 0.007. In 1^st group, the average operation time was 130 min (Q1–Q3 110–140), and in 2^nd group, 150 min (Q1–Q3 115–227.5); p = 0.0159. The average volume of blood loss during laparoscopic zero ischaemia partial nephrectomy was significantly less than during hypotonic partial nephrectomy: 125 ml (Q1–Q350–200) and 450 ml (Q1–Q3200–750) respectively, p <0.0001. Conclusion. In our study, laparoscopic normotonic zero ischaemia partial nephrectomy proved to be a possible alternative to existing resection techniques today. But to use this technique in clinical practice, further study and validation is required.