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XANES measurements for studies of adsorbed protein layers at liquid interfaces. / Konovalov, Oleg V.; Novikova, Natalia N.; Kovalchuk, Mikhail V.; Yalovega, Galina E.; Topunov, Alexey F.; Kosmachevskaya, Olga V.; Yurieva, Eleonora A.; Rogachev, Alexander V.; Trigub, Alexander L.; Kremennaya, Maria A.; Borshchevskiy, Valentin I.; Vakhrameev, Daniil D.; Yakunin, Sergey N.

In: Materials, Vol. 13, No. 20, 4635, 02.10.2020, p. 1-12.

Research output: Contribution to journal › Article › peer-review

Harvard

Konovalov, OV, Novikova, NN, Kovalchuk, MV, Yalovega, GE, Topunov, AF, Kosmachevskaya, OV, Yurieva, EA, Rogachev, AV, Trigub, AL, Kremennaya, MA, Borshchevskiy, VI, Vakhrameev, DD & Yakunin, SN 2020, 'XANES measurements for studies of adsorbed protein layers at liquid interfaces', Materials, vol. 13, no. 20, 4635, pp. 1-12. https://doi.org/10.3390/ma13204635

APA

Konovalov, O. V., Novikova, N. N., Kovalchuk, M. V., Yalovega, G. E., Topunov, A. F., Kosmachevskaya, O. V., Yurieva, E. A., Rogachev, A. V., Trigub, A. L., Kremennaya, M. A., Borshchevskiy, V. I., Vakhrameev, D. D., & Yakunin, S. N. (2020). XANES measurements for studies of adsorbed protein layers at liquid interfaces. Materials, 13(20), 1-12. [4635]. https://doi.org/10.3390/ma13204635

Vancouver

Konovalov OV, Novikova NN, Kovalchuk MV, Yalovega GE, Topunov AF, Kosmachevskaya OV et al. XANES measurements for studies of adsorbed protein layers at liquid interfaces. Materials. 2020 Oct 2;13(20):1-12. 4635. https://doi.org/10.3390/ma13204635

Author

Konovalov, Oleg V. ; Novikova, Natalia N. ; Kovalchuk, Mikhail V. ; Yalovega, Galina E. ; Topunov, Alexey F. ; Kosmachevskaya, Olga V. ; Yurieva, Eleonora A. ; Rogachev, Alexander V. ; Trigub, Alexander L. ; Kremennaya, Maria A. ; Borshchevskiy, Valentin I. ; Vakhrameev, Daniil D. ; Yakunin, Sergey N. / XANES measurements for studies of adsorbed protein layers at liquid interfaces. In: Materials. 2020 ; Vol. 13, No. 20. pp. 1-12.

BibTeX

@article{98159f53c58b4797ac7fe8835a4661a3,
title = "XANES measurements for studies of adsorbed protein layers at liquid interfaces",
abstract = "X-ray absorption near edge structure (XANES) spectra for protein layers adsorbed at liquid interfaces in a Langmuir trough have been recorded for the first time. We studied the parkin protein (so-called E3 ubiquitin ligase), which plays an important role in pathogenesis of Parkinson disease. Parkin contains eight Zn binding sites, consisting of cysteine and histidine residues in a tetracoordinated geometry. Zn K-edge XANES spectra were collected in the following two series: under mild radiation condition of measurements (short exposition time) and with high X-ray radiation load. XANES fingerprint analysis was applied to obtain information on ligand environments around zinc ions. Two types of zinc coordination geometry were identified depending on X-ray radiation load. We found that, under mild conditions, local zinc environment in our parkin preparations was very similar to that identified in hemoglobin, treated with a solution of ZnCl2 salt. Under high X-ray radiation load, considerable changes in the zinc site structure were observed; local zinc environment appeared to be almost identical to that defined in Zn-containing enzyme alkaline phosphatase. The formation of a similar metal site in unrelated protein molecules, observed in our experiments, highlights the significance of metal binding templates as essential structural modules in protein macromolecules.",
keywords = "Langmuir trough, Metalloproteins, Protein layers at liquid interface, XANES, Zinc binding sites",
author = "Konovalov, {Oleg V.} and Novikova, {Natalia N.} and Kovalchuk, {Mikhail V.} and Yalovega, {Galina E.} and Topunov, {Alexey F.} and Kosmachevskaya, {Olga V.} and Yurieva, {Eleonora A.} and Rogachev, {Alexander V.} and Trigub, {Alexander L.} and Kremennaya, {Maria A.} and Borshchevskiy, {Valentin I.} and Vakhrameev, {Daniil D.} and Yakunin, {Sergey N.}",
note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = oct,
day = "2",
doi = "10.3390/ma13204635",
language = "English",
volume = "13",
pages = "1--12",
journal = "Materials",
issn = "1996-1944",
publisher = "MDPI AG",
number = "20",

}

RIS

TY - JOUR

T1 - XANES measurements for studies of adsorbed protein layers at liquid interfaces

AU - Konovalov, Oleg V.

AU - Novikova, Natalia N.

AU - Kovalchuk, Mikhail V.

AU - Yalovega, Galina E.

AU - Topunov, Alexey F.

AU - Kosmachevskaya, Olga V.

AU - Yurieva, Eleonora A.

AU - Rogachev, Alexander V.

AU - Trigub, Alexander L.

AU - Kremennaya, Maria A.

AU - Borshchevskiy, Valentin I.

AU - Vakhrameev, Daniil D.

AU - Yakunin, Sergey N.

N1 - Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/10/2

Y1 - 2020/10/2

N2 - X-ray absorption near edge structure (XANES) spectra for protein layers adsorbed at liquid interfaces in a Langmuir trough have been recorded for the first time. We studied the parkin protein (so-called E3 ubiquitin ligase), which plays an important role in pathogenesis of Parkinson disease. Parkin contains eight Zn binding sites, consisting of cysteine and histidine residues in a tetracoordinated geometry. Zn K-edge XANES spectra were collected in the following two series: under mild radiation condition of measurements (short exposition time) and with high X-ray radiation load. XANES fingerprint analysis was applied to obtain information on ligand environments around zinc ions. Two types of zinc coordination geometry were identified depending on X-ray radiation load. We found that, under mild conditions, local zinc environment in our parkin preparations was very similar to that identified in hemoglobin, treated with a solution of ZnCl2 salt. Under high X-ray radiation load, considerable changes in the zinc site structure were observed; local zinc environment appeared to be almost identical to that defined in Zn-containing enzyme alkaline phosphatase. The formation of a similar metal site in unrelated protein molecules, observed in our experiments, highlights the significance of metal binding templates as essential structural modules in protein macromolecules.

AB - X-ray absorption near edge structure (XANES) spectra for protein layers adsorbed at liquid interfaces in a Langmuir trough have been recorded for the first time. We studied the parkin protein (so-called E3 ubiquitin ligase), which plays an important role in pathogenesis of Parkinson disease. Parkin contains eight Zn binding sites, consisting of cysteine and histidine residues in a tetracoordinated geometry. Zn K-edge XANES spectra were collected in the following two series: under mild radiation condition of measurements (short exposition time) and with high X-ray radiation load. XANES fingerprint analysis was applied to obtain information on ligand environments around zinc ions. Two types of zinc coordination geometry were identified depending on X-ray radiation load. We found that, under mild conditions, local zinc environment in our parkin preparations was very similar to that identified in hemoglobin, treated with a solution of ZnCl2 salt. Under high X-ray radiation load, considerable changes in the zinc site structure were observed; local zinc environment appeared to be almost identical to that defined in Zn-containing enzyme alkaline phosphatase. The formation of a similar metal site in unrelated protein molecules, observed in our experiments, highlights the significance of metal binding templates as essential structural modules in protein macromolecules.

KW - Langmuir trough

KW - Metalloproteins

KW - Protein layers at liquid interface

KW - XANES

KW - Zinc binding sites

UR - http://www.scopus.com/inward/record.url?scp=85093848748&partnerID=8YFLogxK

U2 - 10.3390/ma13204635

DO - 10.3390/ma13204635

M3 - Article

AN - SCOPUS:85093848748

VL - 13

SP - 1

EP - 12

JO - Materials

JF - Materials

SN - 1996-1944

IS - 20

M1 - 4635

ER -

ID: 88197538