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WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris. / Wolabu, Tezera W.; Wang, Hui; Tadesse, Dimiru; Zhang, Fei; Behzadirad, Marjan; Tvorogova, Varvara E.; Abdelmageed, Haggag; Liu, Ye; Chen, Naichong; Chen, Jianghua; Allen, Randy D.; Tadege, Million.

In: New Phytologist, Vol. 229, No. 3, 02.2021, p. 1582 - 1597.

Research output: Contribution to journal › Article › peer-review

Harvard

Wolabu, TW, Wang, H, Tadesse, D, Zhang, F, Behzadirad, M, Tvorogova, VE, Abdelmageed, H, Liu, Y, Chen, N, Chen, J, Allen, RD & Tadege, M 2021, 'WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris', New Phytologist, vol. 229, no. 3, pp. 1582 - 1597. https://doi.org/10.1111/nph.16934

APA

Wolabu, T. W., Wang, H., Tadesse, D., Zhang, F., Behzadirad, M., Tvorogova, V. E., Abdelmageed, H., Liu, Y., Chen, N., Chen, J., Allen, R. D., & Tadege, M. (2021). WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris. New Phytologist, 229(3), 1582 - 1597. https://doi.org/10.1111/nph.16934

Vancouver

Wolabu TW, Wang H, Tadesse D, Zhang F, Behzadirad M, Tvorogova VE et al. WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris. New Phytologist. 2021 Feb;229(3):1582 - 1597. https://doi.org/10.1111/nph.16934

Author

Wolabu, Tezera W. ; Wang, Hui ; Tadesse, Dimiru ; Zhang, Fei ; Behzadirad, Marjan ; Tvorogova, Varvara E. ; Abdelmageed, Haggag ; Liu, Ye ; Chen, Naichong ; Chen, Jianghua ; Allen, Randy D. ; Tadege, Million. / WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris. In: New Phytologist. 2021 ; Vol. 229, No. 3. pp. 1582 - 1597.

BibTeX

@article{74c6ae71c50343578b6718e521d3665c,
title = "WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris",
abstract = "WOX family transcription factors regulate multiple developmental programs. The intermediate clade transcriptional activator WOX9 functions together with the modern clade transcriptional repressor WOX genes in embryogenesis and meristems maintenance, but the mechanism of this interaction is unclear. STF and LAM1 are WOX1 orthologs required for leaf blade outgrowth in Medicago truncatula and Nicotiana sylvestris, respectively. Using biochemical methods and genome editing technology, here we show that WOX9 is an abaxial factor and functions antagonistically to STF and LAM1 to regulate leaf blade development. While NsWOX9 ectopic expression enhances the lam1 mutant phenotype, and antisense expression partially rescues the lam1 mutant, both overexpression and knockout of NsWOX9 in N. sylvestris resulted in a range of severe leaf blade distortions, indicating important role in blade development. Our results indicate that direct repression of WOX9 by WUS clade repressor STF/LAM1 is required for correct blade architecture and patterning in M. truncatula and N. sylvestris. These findings suggest that controlling transcriptional activation and repression mechanisms by direct interaction of activator and repressor WOX genes may be required for cell proliferation and differentiation homeostasis, and could be an evolutionarily conserved mechanism for the development of complex and diverse morphology in flowering plants.",
keywords = "cell proliferation, leaf blade development, leaf polarity, Medicago truncatula, Nicotiana sylvestris, STF, WOX genes, WOX9, STEM-CELL REGULATION, EXPRESSION DYNAMICS, ARABIDOPSIS-THALIANA, SHOOT, FLORAL ORGAN DEVELOPMENT, GROWTH, MERISTEM, WUSCHEL, TRANSCRIPTION FACTOR, HOMEOBOX GENE",
author = "Wolabu, {Tezera W.} and Hui Wang and Dimiru Tadesse and Fei Zhang and Marjan Behzadirad and Tvorogova, {Varvara E.} and Haggag Abdelmageed and Ye Liu and Naichong Chen and Jianghua Chen and Allen, {Randy D.} and Million Tadege",
note = "Funding Information: This work was supported by the National Science Foundation (NSF) grant IOS‐1354422, Agriculture and Food Research Initiative Grant no. 2015‐67014‐22888 from the USDA National Institute of Food and Agriculture, and the Agricultural Experiment Station. Work in VET{\textquoteright}s laboratory at St Petersburg State University, Russia, was supported by the Russian Science Foundation project no. 16‐16‐10011 and by grant from the Russian Foundation for Basic Research no. 20‐016‐00124. Publisher Copyright: {\textcopyright} 2020 The Authors. New Phytologist {\textcopyright} 2020 New Phytologist Foundation Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2021",
month = feb,
doi = "10.1111/nph.16934",
language = "English",
volume = "229",
pages = "1582 -- 1597",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris

AU - Wolabu, Tezera W.

AU - Wang, Hui

AU - Tadesse, Dimiru

AU - Zhang, Fei

AU - Behzadirad, Marjan

AU - Tvorogova, Varvara E.

AU - Abdelmageed, Haggag

AU - Liu, Ye

AU - Chen, Naichong

AU - Chen, Jianghua

AU - Allen, Randy D.

AU - Tadege, Million

N1 - Funding Information: This work was supported by the National Science Foundation (NSF) grant IOS‐1354422, Agriculture and Food Research Initiative Grant no. 2015‐67014‐22888 from the USDA National Institute of Food and Agriculture, and the Agricultural Experiment Station. Work in VET’s laboratory at St Petersburg State University, Russia, was supported by the Russian Science Foundation project no. 16‐16‐10011 and by grant from the Russian Foundation for Basic Research no. 20‐016‐00124. Publisher Copyright: © 2020 The Authors. New Phytologist © 2020 New Phytologist Foundation Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2021/2

Y1 - 2021/2

N2 - WOX family transcription factors regulate multiple developmental programs. The intermediate clade transcriptional activator WOX9 functions together with the modern clade transcriptional repressor WOX genes in embryogenesis and meristems maintenance, but the mechanism of this interaction is unclear. STF and LAM1 are WOX1 orthologs required for leaf blade outgrowth in Medicago truncatula and Nicotiana sylvestris, respectively. Using biochemical methods and genome editing technology, here we show that WOX9 is an abaxial factor and functions antagonistically to STF and LAM1 to regulate leaf blade development. While NsWOX9 ectopic expression enhances the lam1 mutant phenotype, and antisense expression partially rescues the lam1 mutant, both overexpression and knockout of NsWOX9 in N. sylvestris resulted in a range of severe leaf blade distortions, indicating important role in blade development. Our results indicate that direct repression of WOX9 by WUS clade repressor STF/LAM1 is required for correct blade architecture and patterning in M. truncatula and N. sylvestris. These findings suggest that controlling transcriptional activation and repression mechanisms by direct interaction of activator and repressor WOX genes may be required for cell proliferation and differentiation homeostasis, and could be an evolutionarily conserved mechanism for the development of complex and diverse morphology in flowering plants.

AB - WOX family transcription factors regulate multiple developmental programs. The intermediate clade transcriptional activator WOX9 functions together with the modern clade transcriptional repressor WOX genes in embryogenesis and meristems maintenance, but the mechanism of this interaction is unclear. STF and LAM1 are WOX1 orthologs required for leaf blade outgrowth in Medicago truncatula and Nicotiana sylvestris, respectively. Using biochemical methods and genome editing technology, here we show that WOX9 is an abaxial factor and functions antagonistically to STF and LAM1 to regulate leaf blade development. While NsWOX9 ectopic expression enhances the lam1 mutant phenotype, and antisense expression partially rescues the lam1 mutant, both overexpression and knockout of NsWOX9 in N. sylvestris resulted in a range of severe leaf blade distortions, indicating important role in blade development. Our results indicate that direct repression of WOX9 by WUS clade repressor STF/LAM1 is required for correct blade architecture and patterning in M. truncatula and N. sylvestris. These findings suggest that controlling transcriptional activation and repression mechanisms by direct interaction of activator and repressor WOX genes may be required for cell proliferation and differentiation homeostasis, and could be an evolutionarily conserved mechanism for the development of complex and diverse morphology in flowering plants.

KW - cell proliferation

KW - leaf blade development

KW - leaf polarity

KW - Medicago truncatula

KW - Nicotiana sylvestris

KW - STF

KW - WOX genes

KW - WOX9

KW - STEM-CELL REGULATION

KW - EXPRESSION DYNAMICS

KW - ARABIDOPSIS-THALIANA

KW - SHOOT

KW - FLORAL ORGAN DEVELOPMENT

KW - GROWTH

KW - MERISTEM

KW - WUSCHEL

KW - TRANSCRIPTION FACTOR

KW - HOMEOBOX GENE

UR - http://www.scopus.com/inward/record.url?scp=85092726952&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d00e9af2-5464-3402-9380-5373faa741a4/

U2 - 10.1111/nph.16934

DO - 10.1111/nph.16934

M3 - Article

AN - SCOPUS:85092726952

VL - 229

SP - 1582

EP - 1597

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 3

ER -

ID: 71622699