The article describes the influence of the work experience of the administrative employees of the higher educational institutions on the perceived significance of knowledge sharing barriers faced by them in their work activities. The methodology suggests testing the relationship between the work experience and the 12 knowledge sharing barriers perceived significance (estimated from the positions of the respondent as a knowledge requester and a knowledge holder, thus giving 24 equations) by linear regression modeling. The equations with statistically significant work experience coefficient contain the perceived significance of the knowledge sharing barrier of requested knowledge sensitivity for its holder, both for the respondents' knowledge requester and holder position. On the basis of the educed results, recommendations are given to managers of the higher educational institutions administrative subdivisions regarding mitigating the influence of the barrier of the requested knowledge importance for its holder, especially considering its influence on the employees with smaller work experience. Several recommendations to business practitioners outside of the higher educational institutions are also given, regarding collaboration with such institutions' administrative subdivisions. This article develops the inquiry into the knowledge sharing barriers problem in the higher educational institutions administrative subdivisions, that have not been a specific object of research in knowledge sharing barriers before the research project including the research presented in the current article. A number of further research directions is also suggested.