Water-soluble multimode fluorescent thermometers based on porphyrins photosensitizers. / Kolesnikov, Ilya E.; Kurochkin, Mikhail A.; Meshkov, Ivan N.; Akasov, Roman A.; Kalinichev, Alexey A.; Kolesnikov, Evgenii Yu; Gorbunova, Yulia G.; Lähderanta, Erkki.
In: Materials and Design, Vol. 203, 109613, 01.05.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Water-soluble multimode fluorescent thermometers based on porphyrins photosensitizers
AU - Kolesnikov, Ilya E.
AU - Kurochkin, Mikhail A.
AU - Meshkov, Ivan N.
AU - Akasov, Roman A.
AU - Kalinichev, Alexey A.
AU - Kolesnikov, Evgenii Yu
AU - Gorbunova, Yulia G.
AU - Lähderanta, Erkki
N1 - Publisher Copyright: © 2021 The Authors
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Anti-cancer treatment efficiency can be significantly enhanced by the synergistic effect of the combined phototherapy that nowadays is under intensive preclinical and clinical studies. To minimize collateral damage, the combined phototherapy should be accompanied by a real-time thermal sensing. However, it is still challenging to develop multifunctional agent which combines therapy and temperature control. In the present study, contactless fluorescent thermometry based on ratiometric and lifetime approaches was realized using water-soluble anionic meso-tetrasulfonatophenylporphyrinate (tetra sodium salt) TSPH2 and cationic meso-mono(4-pyridyl)-triphenylporphyrinato)phosphorus(V) bromide MPyPP(OH)2 porphyrins photosensitizers. Both sensing techniques provide simple linear calibration curves in the biological temperature range. Thermometric performance of the suggested porphyrins was evaluated in terms of relative sensitivity, temperature resolution and repeatability and was found to be in upper range among porphyrin-based thermometers reaching 1% K−1 and 0.1 °C. The relative sensitivity was enhanced from 2 to 7 times by using peak-to-valley ratio compared to usual peak-to-peak ratio. Porphyrins' cytotoxicity was studied in vitro both in dark and blue-light activated conditions. Exposure-dependent cell viability showed that porphyrins could be used as molecular thermometers for phototherapy to avoid heat-induced adverse effects or as promising photosensitizers depending on chosen laser power.
AB - Anti-cancer treatment efficiency can be significantly enhanced by the synergistic effect of the combined phototherapy that nowadays is under intensive preclinical and clinical studies. To minimize collateral damage, the combined phototherapy should be accompanied by a real-time thermal sensing. However, it is still challenging to develop multifunctional agent which combines therapy and temperature control. In the present study, contactless fluorescent thermometry based on ratiometric and lifetime approaches was realized using water-soluble anionic meso-tetrasulfonatophenylporphyrinate (tetra sodium salt) TSPH2 and cationic meso-mono(4-pyridyl)-triphenylporphyrinato)phosphorus(V) bromide MPyPP(OH)2 porphyrins photosensitizers. Both sensing techniques provide simple linear calibration curves in the biological temperature range. Thermometric performance of the suggested porphyrins was evaluated in terms of relative sensitivity, temperature resolution and repeatability and was found to be in upper range among porphyrin-based thermometers reaching 1% K−1 and 0.1 °C. The relative sensitivity was enhanced from 2 to 7 times by using peak-to-valley ratio compared to usual peak-to-peak ratio. Porphyrins' cytotoxicity was studied in vitro both in dark and blue-light activated conditions. Exposure-dependent cell viability showed that porphyrins could be used as molecular thermometers for phototherapy to avoid heat-induced adverse effects or as promising photosensitizers depending on chosen laser power.
KW - Cytotoxicity
KW - Fluorescent thermometry
KW - Lifetime
KW - Sensor
KW - Water-soluble porphyrin
KW - THERMAL SENSORS
KW - PHOTODYNAMIC THERAPY
KW - TEMPERATURE
KW - KINETICS
KW - MOLECULAR THERMOMETER
KW - SINGLET OXYGEN
KW - ABSORPTION
KW - SPECTRA
KW - EMISSION
KW - EFFICIENCY
UR - http://www.scopus.com/inward/record.url?scp=85101775296&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/de230bef-9705-3b4f-9ca5-ea9e553a0811/
U2 - 10.1016/j.matdes.2021.109613
DO - 10.1016/j.matdes.2021.109613
M3 - Article
AN - SCOPUS:85101775296
VL - 203
JO - Materials and Design
JF - Materials and Design
SN - 0261-3069
M1 - 109613
ER -
ID: 86367120