TY - JOUR

T1 - Water-soluble multimode fluorescent thermometers based on porphyrins photosensitizers

AU - Kolesnikov, Ilya E.

AU - Kurochkin, Mikhail A.

AU - Meshkov, Ivan N.

AU - Akasov, Roman A.

AU - Kalinichev, Alexey A.

AU - Kolesnikov, Evgenii Yu

AU - Gorbunova, Yulia G.

AU - Lähderanta, Erkki

N1 - Publisher Copyright: © 2021 The Authors

PY - 2021/5/1

Y1 - 2021/5/1

N2 - Anti-cancer treatment efficiency can be significantly enhanced by the synergistic effect of the combined phototherapy that nowadays is under intensive preclinical and clinical studies. To minimize collateral damage, the combined phototherapy should be accompanied by a real-time thermal sensing. However, it is still challenging to develop multifunctional agent which combines therapy and temperature control. In the present study, contactless fluorescent thermometry based on ratiometric and lifetime approaches was realized using water-soluble anionic meso-tetrasulfonatophenylporphyrinate (tetra sodium salt) TSPH2 and cationic meso-mono(4-pyridyl)-triphenylporphyrinato)phosphorus(V) bromide MPyPP(OH)2 porphyrins photosensitizers. Both sensing techniques provide simple linear calibration curves in the biological temperature range. Thermometric performance of the suggested porphyrins was evaluated in terms of relative sensitivity, temperature resolution and repeatability and was found to be in upper range among porphyrin-based thermometers reaching 1% K−1 and 0.1 °C. The relative sensitivity was enhanced from 2 to 7 times by using peak-to-valley ratio compared to usual peak-to-peak ratio. Porphyrins' cytotoxicity was studied in vitro both in dark and blue-light activated conditions. Exposure-dependent cell viability showed that porphyrins could be used as molecular thermometers for phototherapy to avoid heat-induced adverse effects or as promising photosensitizers depending on chosen laser power.

AB - Anti-cancer treatment efficiency can be significantly enhanced by the synergistic effect of the combined phototherapy that nowadays is under intensive preclinical and clinical studies. To minimize collateral damage, the combined phototherapy should be accompanied by a real-time thermal sensing. However, it is still challenging to develop multifunctional agent which combines therapy and temperature control. In the present study, contactless fluorescent thermometry based on ratiometric and lifetime approaches was realized using water-soluble anionic meso-tetrasulfonatophenylporphyrinate (tetra sodium salt) TSPH2 and cationic meso-mono(4-pyridyl)-triphenylporphyrinato)phosphorus(V) bromide MPyPP(OH)2 porphyrins photosensitizers. Both sensing techniques provide simple linear calibration curves in the biological temperature range. Thermometric performance of the suggested porphyrins was evaluated in terms of relative sensitivity, temperature resolution and repeatability and was found to be in upper range among porphyrin-based thermometers reaching 1% K−1 and 0.1 °C. The relative sensitivity was enhanced from 2 to 7 times by using peak-to-valley ratio compared to usual peak-to-peak ratio. Porphyrins' cytotoxicity was studied in vitro both in dark and blue-light activated conditions. Exposure-dependent cell viability showed that porphyrins could be used as molecular thermometers for phototherapy to avoid heat-induced adverse effects or as promising photosensitizers depending on chosen laser power.

KW - Cytotoxicity

KW - Fluorescent thermometry

KW - Lifetime

KW - Sensor

KW - Water-soluble porphyrin

KW - THERMAL SENSORS

KW - PHOTODYNAMIC THERAPY

KW - TEMPERATURE

KW - KINETICS

KW - MOLECULAR THERMOMETER

KW - SINGLET OXYGEN

KW - ABSORPTION

KW - SPECTRA

KW - EMISSION

KW - EFFICIENCY

UR - http://www.scopus.com/inward/record.url?scp=85101775296&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/de230bef-9705-3b4f-9ca5-ea9e553a0811/

U2 - 10.1016/j.matdes.2021.109613

DO - 10.1016/j.matdes.2021.109613

M3 - Article

AN - SCOPUS:85101775296

VL - 203

JO - Materials and Design

JF - Materials and Design

SN - 0261-3069

M1 - 109613

ER -