Effect of factors of sealed non-aired space on the organism leads to the enhancement of catabolism of L-4-[14C]-aspartate in the mice encephalon to 14CO2. Preliminary administration of L-aspartate to the organism (100 mg/kg) leads to the intensification of these adaptative reactions and prolongs life of animals under these conditions. Accumulation of the introduced aspartate in the liver and encephalon gets more intensive and its supply to the blood is more rapid under these conditions. Simultaneous (together with L-aspartate) administration of the vitamin-coenzyme complex (pentapyruvate) which includes thiamine pyrophosphate, lipoate, sodium 4-phosphopantotenate, nicotinate and riboflavin-mononucleotide and stimulates the function of the key links of the Krebs cycle to animals has induced intensification of the protective effect of L-aspartate and evoked further activation of the L-aspartate catabolism in the encephalon, mainly at late, preagonal stages of the developing pathological state. The data presented confirm that protective effect of L-aspartate, provided the effect of the closed space factors on the organism, is a result of its quick introduction, into energy mebolism of tissues, in particular, of the nervous tissue.