Standard

Viral infiltration of pancreatic islets in patients with COVID-19. / Steenblock, Charlotte; Richter, Stefanie; Berger, Ilona; Barovic, Marko; Schmid, Janine; Schubert, Undine; Jarzebska, Natalia; von Mässenhausen, Anne; Linkermann, Andreas; Schürmann, Annette; Pablik, Jessica; Dienemann, Thomas; Evert, Katja; Rodionov, Roman N.; Semenova, Natalia Y.; Zinserling, Vsevolod A.; Gainetdinov, Raul R.; Baretton, Gustavo; Lindemann, Dirk; Solimena, Michele; Ludwig, Barbara; Bornstein, Stefan R.

In: Nature Communications, Vol. 12, No. 1, 3534, 10.06.2021.

Research output: Contribution to journal › Article › peer-review

Harvard

Steenblock, C, Richter, S, Berger, I, Barovic, M, Schmid, J, Schubert, U, Jarzebska, N, von Mässenhausen, A, Linkermann, A, Schürmann, A, Pablik, J, Dienemann, T, Evert, K, Rodionov, RN, Semenova, NY, Zinserling, VA, Gainetdinov, RR, Baretton, G, Lindemann, D, Solimena, M, Ludwig, B & Bornstein, SR 2021, 'Viral infiltration of pancreatic islets in patients with COVID-19', Nature Communications, vol. 12, no. 1, 3534. https://doi.org/10.1038/s41467-021-23886-3

APA

Steenblock, C., Richter, S., Berger, I., Barovic, M., Schmid, J., Schubert, U., Jarzebska, N., von Mässenhausen, A., Linkermann, A., Schürmann, A., Pablik, J., Dienemann, T., Evert, K., Rodionov, R. N., Semenova, N. Y., Zinserling, V. A., Gainetdinov, R. R., Baretton, G., Lindemann, D., ... Bornstein, S. R. (2021). Viral infiltration of pancreatic islets in patients with COVID-19. Nature Communications, 12(1), [3534]. https://doi.org/10.1038/s41467-021-23886-3

Vancouver

Steenblock C, Richter S, Berger I, Barovic M, Schmid J, Schubert U et al. Viral infiltration of pancreatic islets in patients with COVID-19. Nature Communications. 2021 Jun 10;12(1). 3534. https://doi.org/10.1038/s41467-021-23886-3

Author

Steenblock, Charlotte ; Richter, Stefanie ; Berger, Ilona ; Barovic, Marko ; Schmid, Janine ; Schubert, Undine ; Jarzebska, Natalia ; von Mässenhausen, Anne ; Linkermann, Andreas ; Schürmann, Annette ; Pablik, Jessica ; Dienemann, Thomas ; Evert, Katja ; Rodionov, Roman N. ; Semenova, Natalia Y. ; Zinserling, Vsevolod A. ; Gainetdinov, Raul R. ; Baretton, Gustavo ; Lindemann, Dirk ; Solimena, Michele ; Ludwig, Barbara ; Bornstein, Stefan R. / Viral infiltration of pancreatic islets in patients with COVID-19. In: Nature Communications. 2021 ; Vol. 12, No. 1.

BibTeX

@article{c5d82d9471d64efb8879ec143eb3a09b,
title = "Viral infiltration of pancreatic islets in patients with COVID-19",
abstract = "Metabolic diseases are associated with an increased risk of severe COVID-19 and conversely, new-onset hyperglycemia and complications of preexisting diabetes have been observed in COVID-19 patients. Here, we performed a comprehensive analysis of pancreatic autopsy tissue from COVID-19 patients using immunofluorescence, immunohistochemistry, RNA scope and electron microscopy and detected SARS-CoV-2 viral infiltration of beta-cells in all patients. Using SARS-CoV-2 pseudoviruses, we confirmed that isolated human islet cells are permissive to infection. In eleven COVID-19 patients, we examined the expression of ACE2, TMPRSS and other receptors and factors, such as DPP4, HMBG1 and NRP1, that might facilitate virus entry. Whereas 70% of the COVID-19 patients expressed ACE2 in the vasculature, only 30% displayed ACE2-expression in beta-cells. Even in the absence of manifest new-onset diabetes, necroptotic cell death, immune cell infiltration and SARS-CoV-2 viral infection of pancreatic beta-cells may contribute to varying degrees of metabolic dysregulation in patients with COVID-19.",
keywords = "Adult, Aged, Angiotensin-Converting Enzyme 2/metabolism, Autopsy, COVID-19/pathology, Diabetes Complications/pathology, Diabetes Mellitus/pathology, Dipeptidyl Peptidase 4/metabolism, Female, HMGN Proteins/metabolism, Humans, Insulin-Secreting Cells/metabolism, Male, Middle Aged, Neuropilin-1/metabolism, Organ Specificity/physiology, Receptors, Coronavirus/metabolism, SARS-CoV-2/isolation & purification, Serine Endopeptidases/metabolism, SYSTEM, TMPRSS2, DOWNSTREAM, NECROPTOSIS, MLKL, ACE2, EXPRESSION, ENTRY FACTORS",
author = "Charlotte Steenblock and Stefanie Richter and Ilona Berger and Marko Barovic and Janine Schmid and Undine Schubert and Natalia Jarzebska and {von M{\"a}ssenhausen}, Anne and Andreas Linkermann and Annette Sch{\"u}rmann and Jessica Pablik and Thomas Dienemann and Katja Evert and Rodionov, {Roman N.} and Semenova, {Natalia Y.} and Zinserling, {Vsevolod A.} and Gainetdinov, {Raul R.} and Gustavo Baretton and Dirk Lindemann and Michele Solimena and Barbara Ludwig and Bornstein, {Stefan R.}",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = jun,
day = "10",
doi = "10.1038/s41467-021-23886-3",
language = "English",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Viral infiltration of pancreatic islets in patients with COVID-19

AU - Steenblock, Charlotte

AU - Richter, Stefanie

AU - Berger, Ilona

AU - Barovic, Marko

AU - Schmid, Janine

AU - Schubert, Undine

AU - Jarzebska, Natalia

AU - von Mässenhausen, Anne

AU - Linkermann, Andreas

AU - Schürmann, Annette

AU - Pablik, Jessica

AU - Dienemann, Thomas

AU - Evert, Katja

AU - Rodionov, Roman N.

AU - Semenova, Natalia Y.

AU - Zinserling, Vsevolod A.

AU - Gainetdinov, Raul R.

AU - Baretton, Gustavo

AU - Lindemann, Dirk

AU - Solimena, Michele

AU - Ludwig, Barbara

AU - Bornstein, Stefan R.

N1 - Publisher Copyright: © 2021, The Author(s).

PY - 2021/6/10

Y1 - 2021/6/10

N2 - Metabolic diseases are associated with an increased risk of severe COVID-19 and conversely, new-onset hyperglycemia and complications of preexisting diabetes have been observed in COVID-19 patients. Here, we performed a comprehensive analysis of pancreatic autopsy tissue from COVID-19 patients using immunofluorescence, immunohistochemistry, RNA scope and electron microscopy and detected SARS-CoV-2 viral infiltration of beta-cells in all patients. Using SARS-CoV-2 pseudoviruses, we confirmed that isolated human islet cells are permissive to infection. In eleven COVID-19 patients, we examined the expression of ACE2, TMPRSS and other receptors and factors, such as DPP4, HMBG1 and NRP1, that might facilitate virus entry. Whereas 70% of the COVID-19 patients expressed ACE2 in the vasculature, only 30% displayed ACE2-expression in beta-cells. Even in the absence of manifest new-onset diabetes, necroptotic cell death, immune cell infiltration and SARS-CoV-2 viral infection of pancreatic beta-cells may contribute to varying degrees of metabolic dysregulation in patients with COVID-19.

AB - Metabolic diseases are associated with an increased risk of severe COVID-19 and conversely, new-onset hyperglycemia and complications of preexisting diabetes have been observed in COVID-19 patients. Here, we performed a comprehensive analysis of pancreatic autopsy tissue from COVID-19 patients using immunofluorescence, immunohistochemistry, RNA scope and electron microscopy and detected SARS-CoV-2 viral infiltration of beta-cells in all patients. Using SARS-CoV-2 pseudoviruses, we confirmed that isolated human islet cells are permissive to infection. In eleven COVID-19 patients, we examined the expression of ACE2, TMPRSS and other receptors and factors, such as DPP4, HMBG1 and NRP1, that might facilitate virus entry. Whereas 70% of the COVID-19 patients expressed ACE2 in the vasculature, only 30% displayed ACE2-expression in beta-cells. Even in the absence of manifest new-onset diabetes, necroptotic cell death, immune cell infiltration and SARS-CoV-2 viral infection of pancreatic beta-cells may contribute to varying degrees of metabolic dysregulation in patients with COVID-19.

KW - Adult

KW - Aged

KW - Angiotensin-Converting Enzyme 2/metabolism

KW - Autopsy

KW - COVID-19/pathology

KW - Diabetes Complications/pathology

KW - Diabetes Mellitus/pathology

KW - Dipeptidyl Peptidase 4/metabolism

KW - Female

KW - HMGN Proteins/metabolism

KW - Humans

KW - Insulin-Secreting Cells/metabolism

KW - Male

KW - Middle Aged

KW - Neuropilin-1/metabolism

KW - Organ Specificity/physiology

KW - Receptors, Coronavirus/metabolism

KW - SARS-CoV-2/isolation & purification

KW - Serine Endopeptidases/metabolism

KW - SYSTEM

KW - TMPRSS2

KW - DOWNSTREAM

KW - NECROPTOSIS

KW - MLKL

KW - ACE2

KW - EXPRESSION

KW - ENTRY FACTORS

UR - http://www.scopus.com/inward/record.url?scp=85107527595&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/76ed6caa-9f04-3f2a-8488-913038be060e/

U2 - 10.1038/s41467-021-23886-3

DO - 10.1038/s41467-021-23886-3

M3 - Article

C2 - 34112801

AN - SCOPUS:85107527595

VL - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3534

ER -

ID: 87888221