Vesicle shrinking and enlargement: the yin and yang of exocytotic content release : Yin and yang of fusion modes in control of release. / Shin, Wonchul; Arpino, Gianvito; Thiyagarajan, Sathish; Su, Rui; McDargh, Zachary ; Ge, Lihao; Guo, Xiaoli; Wei, Lisi; Shupliakov, Oleg ; Jin, Albert; O'Shaughnessy, Ben; Wu, Ling Gang.
In: Biophysical Journal, Vol. 118, No. 3 S1, 399A, 07.02.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Vesicle shrinking and enlargement: the yin and yang of exocytotic content release
T2 - Yin and yang of fusion modes in control of release
AU - Shin, Wonchul
AU - Arpino, Gianvito
AU - Thiyagarajan, Sathish
AU - Su, Rui
AU - McDargh, Zachary
AU - Ge, Lihao
AU - Guo, Xiaoli
AU - Wei, Lisi
AU - Shupliakov, Oleg
AU - Jin, Albert
AU - O'Shaughnessy, Ben
AU - Wu, Ling Gang
PY - 2020/2/7
Y1 - 2020/2/7
N2 - For decades, two fusion modes were thought to control hormone and transmitter release essential to life: one facilitates release via fusion pore dilation and flattening (full-collapse), the other limits release by closing a narrow fusion pore (kiss-and-run). Using super-resolution STED microscopy to visualize fusion modes of dense-core vesicles in neuroendocrine cells, we found that facilitation of release was not mediated by full-collapse, but shrink-fusion, in which the Ω-profile generated by vesicle fusion shrank, but maintained a large non-dilating pore. We discovered that cells’ physiological osmotic pressure squeezed, but not dilated the Ω-profile, explaining why shrink-fusion prevailed over full-collapse. Instead of kiss-and-run, enlarge-fusion, in which Ω-profiles grew while maintaining a narrow pore, slowed down release. Shrink- and enlarge-fusion may thus be the yin and yang of fusion modes that contribute to account for diverse hormone and transmitter release kinetics observed in secretory cells, previously interpreted within the full-collapse/kiss-and-run framework.
AB - For decades, two fusion modes were thought to control hormone and transmitter release essential to life: one facilitates release via fusion pore dilation and flattening (full-collapse), the other limits release by closing a narrow fusion pore (kiss-and-run). Using super-resolution STED microscopy to visualize fusion modes of dense-core vesicles in neuroendocrine cells, we found that facilitation of release was not mediated by full-collapse, but shrink-fusion, in which the Ω-profile generated by vesicle fusion shrank, but maintained a large non-dilating pore. We discovered that cells’ physiological osmotic pressure squeezed, but not dilated the Ω-profile, explaining why shrink-fusion prevailed over full-collapse. Instead of kiss-and-run, enlarge-fusion, in which Ω-profiles grew while maintaining a narrow pore, slowed down release. Shrink- and enlarge-fusion may thus be the yin and yang of fusion modes that contribute to account for diverse hormone and transmitter release kinetics observed in secretory cells, previously interpreted within the full-collapse/kiss-and-run framework.
KW - Exocytosis
KW - transmitter release
KW - hormone release
KW - osmotic pressure
KW - membrane dynamics
KW - membrane fusion
KW - vesicle shrinking
KW - vesicle enlargement
KW - bovine chromaffin cells
UR - https://www.cell.com/biophysj/fulltext/S0006-3495(19)33199-6#%20
M3 - Article
VL - 118
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 3 S1
M1 - 399A
ER -
ID: 43670718