Vanadium-dependent haloperoxidase activity and phlorotannin incorporation into the cell wall during early embryogenesis of Fucus vesiculosus (Phaeophyceae)

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Vanadium-dependent haloperoxidase activity and phlorotannin incorporation into the cell wall during early embryogenesis of Fucus vesiculosus (Phaeophyceae). / Lemesheva, Valeriya; Birkemeyer, Claudia; Garbary, David; Tarakhovskaya, Elena.

In: European Journal of Phycology, Vol. 55, No. 3, 02.07.2020, p. 275-284.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{7c3164bd80114f38904dcef315596acf,

title = "Vanadium-dependent haloperoxidase activity and phlorotannin incorporation into the cell wall during early embryogenesis of Fucus vesiculosus (Phaeophyceae)",

abstract = "Vanadium-dependent haloperoxidases (V-HPO) are specific enzymes involved in the metabolism of reactive oxygen species in marine algae. These enzymes contribute to early embryogenesis of algae in the order Fucales, catalyzing the reactions which result in phlorotannin incorporation into the newly forming zygote cell wall and adhesive material. In this study we showed that early embryogenesis in Fucus vesiculosus is accompanied by synchronous changes of V-HPO activity, hydrogen peroxide content and content of cell-wall-associated phlorotannins. Phlorotannin incorporation into the cell wall of Fucus zygotes had already begun 1 h after fertilization, and this process was accompanied by rapid increase of H2O2 content and V-HPO activity. We suggest that altogether this initiates polymerization of phenolics and their cross-linking with the major cell wall constituent, alginic acid. Presumably, de novo phlorotannin biosynthesis is involved in this process, as GC-MS analysis showed dramatic changes in content of intracellular phloroglucinol and low-molecular-weight phlorotannins during the first 9 days of F. vesiculosus embryogenesis.",

keywords = "Brown algae, cell wall, embryogenesis, Fucus, haloperoxidase, hydrogen peroxide, phloroglucinol, phlorotannins, zygote, PHENOLIC SUBSTANCES, XYLENOL ORANGE, PHOTOSYNTHETIC APPARATUS, FUCALES, ZYGOTES, INDUCTION, EXTRACTION, FLUORESCENCE, BROMOPEROXIDASE, EMBRYOS",

author = "Valeriya Lemesheva and Claudia Birkemeyer and David Garbary and Elena Tarakhovskaya",

note = "Publisher Copyright: {\textcopyright} 2020, {\textcopyright} 2020 British Phycological Society.",

year = "2020",

month = jul,

day = "2",

doi = "10.1080/09670262.2019.1709131",

language = "English",

volume = "55",

pages = "275--284",

journal = "European Journal of Phycology",

issn = "0967-0262",

publisher = "Taylor & Francis",

number = "3",

}

RIS

TY - JOUR

T1 - Vanadium-dependent haloperoxidase activity and phlorotannin incorporation into the cell wall during early embryogenesis of Fucus vesiculosus (Phaeophyceae)

AU - Lemesheva, Valeriya

AU - Birkemeyer, Claudia

AU - Garbary, David

AU - Tarakhovskaya, Elena

PY - 2020/7/2

Y1 - 2020/7/2

N2 - Vanadium-dependent haloperoxidases (V-HPO) are specific enzymes involved in the metabolism of reactive oxygen species in marine algae. These enzymes contribute to early embryogenesis of algae in the order Fucales, catalyzing the reactions which result in phlorotannin incorporation into the newly forming zygote cell wall and adhesive material. In this study we showed that early embryogenesis in Fucus vesiculosus is accompanied by synchronous changes of V-HPO activity, hydrogen peroxide content and content of cell-wall-associated phlorotannins. Phlorotannin incorporation into the cell wall of Fucus zygotes had already begun 1 h after fertilization, and this process was accompanied by rapid increase of H2O2 content and V-HPO activity. We suggest that altogether this initiates polymerization of phenolics and their cross-linking with the major cell wall constituent, alginic acid. Presumably, de novo phlorotannin biosynthesis is involved in this process, as GC-MS analysis showed dramatic changes in content of intracellular phloroglucinol and low-molecular-weight phlorotannins during the first 9 days of F. vesiculosus embryogenesis.

AB - Vanadium-dependent haloperoxidases (V-HPO) are specific enzymes involved in the metabolism of reactive oxygen species in marine algae. These enzymes contribute to early embryogenesis of algae in the order Fucales, catalyzing the reactions which result in phlorotannin incorporation into the newly forming zygote cell wall and adhesive material. In this study we showed that early embryogenesis in Fucus vesiculosus is accompanied by synchronous changes of V-HPO activity, hydrogen peroxide content and content of cell-wall-associated phlorotannins. Phlorotannin incorporation into the cell wall of Fucus zygotes had already begun 1 h after fertilization, and this process was accompanied by rapid increase of H2O2 content and V-HPO activity. We suggest that altogether this initiates polymerization of phenolics and their cross-linking with the major cell wall constituent, alginic acid. Presumably, de novo phlorotannin biosynthesis is involved in this process, as GC-MS analysis showed dramatic changes in content of intracellular phloroglucinol and low-molecular-weight phlorotannins during the first 9 days of F. vesiculosus embryogenesis.

KW - Brown algae

KW - cell wall

KW - embryogenesis

KW - Fucus

KW - haloperoxidase

KW - hydrogen peroxide

KW - phloroglucinol

KW - phlorotannins

KW - zygote

KW - PHENOLIC SUBSTANCES

KW - XYLENOL ORANGE

KW - PHOTOSYNTHETIC APPARATUS

KW - FUCALES

KW - ZYGOTES

KW - INDUCTION

KW - EXTRACTION

KW - FLUORESCENCE

KW - BROMOPEROXIDASE

KW - EMBRYOS

UR - http://www.scopus.com/inward/record.url?scp=85080874574&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/04ed67bf-2c73-3f9b-b392-be8531d0b2fd/

U2 - 10.1080/09670262.2019.1709131

DO - 10.1080/09670262.2019.1709131

M3 - Article

AN - SCOPUS:85080874574

VL - 55

SP - 275

EP - 284

JO - European Journal of Phycology

JF - European Journal of Phycology

SN - 0967-0262

IS - 3

ER -

ID: 52292137