The multi-agent approach in the study of university learning environment opens up the prospect of constructive analysis of the different information interconnections in process professional training specialist. The authors aim to identify criteria for assessing the quality of the learning environment and meaningful motivations of participants in the educational process. E-learning at a level mediated by computer software technology is accompanied by a transformation of the established system of relationships of traditional participants in the educational process. Students, teachers, leaders who enter into interactions within the educational system of the university have different goals that determine their attitude to the E-learning environment. The distance E-learning format adds a new level of communication to the student–teacher relationship system, which has its own technological and semantic features and creates barriers to information and cognitive exchange. In this regard, the study of the university educational environment as a multi-agent system and comparative analysis of the positions of its participants are relevant. The study is based on a methodology of system approach. The empirical base is compiled by survey data of representative groups, obtained by the method of online and offline questionnaires. The research results can serve as a basis for improving the educational policy of the university and technologies for information support of the educational process. The revealed tendencies of target coincidence and problem points in information exchange agents interaction are a tool for predicting changes in the university electronic environment to increase the effectiveness of professional training of a qualified specialist.