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Transcriptome analysis unveils the effects of proline on gene expression in the yeast komagataella phaffii. / Rumyantsev, Andrey; Sidorin, Anton; Volkov, Artemii; Shanaa, Ousama Al; Sambuk, Elena; Padkina, Marina.

In: Microorganisms, Vol. 10, No. 1, 67, 01.2022.

Research output: Contribution to journal › Article › peer-review

Harvard

Rumyantsev, A, Sidorin, A, Volkov, A, Shanaa, OA, Sambuk, E & Padkina, M 2022, 'Transcriptome analysis unveils the effects of proline on gene expression in the yeast komagataella phaffii', Microorganisms, vol. 10, no. 1, 67. https://doi.org/10.3390/microorganisms10010067

APA

Rumyantsev, A., Sidorin, A., Volkov, A., Shanaa, O. A., Sambuk, E., & Padkina, M. (2022). Transcriptome analysis unveils the effects of proline on gene expression in the yeast komagataella phaffii. Microorganisms, 10(1), [67]. https://doi.org/10.3390/microorganisms10010067

Vancouver

Rumyantsev A, Sidorin A, Volkov A, Shanaa OA, Sambuk E, Padkina M. Transcriptome analysis unveils the effects of proline on gene expression in the yeast komagataella phaffii. Microorganisms. 2022 Jan;10(1). 67. https://doi.org/10.3390/microorganisms10010067

Author

Rumyantsev, Andrey ; Sidorin, Anton ; Volkov, Artemii ; Shanaa, Ousama Al ; Sambuk, Elena ; Padkina, Marina. / Transcriptome analysis unveils the effects of proline on gene expression in the yeast komagataella phaffii. In: Microorganisms. 2022 ; Vol. 10, No. 1.

BibTeX

@article{c2a9a0e16d8c42a68d6e74e16ea8cb13,
title = "Transcriptome analysis unveils the effects of proline on gene expression in the yeast komagataella phaffii",
abstract = "Komagataella phaffii yeast is one of the most important biocompounds producing micro-organisms in modern biotechnology. Optimization of media recipes and cultivation strategies is key to successful synthesis of recombinant proteins. The complex effects of proline on gene expression in the yeast K. phaffii was analyzed on the transcriptome level in this work. Our analysis revealed drastic changes in gene expression when K. phaffii was grown in proline‐containing media in comparison to ammonium sulphate‐containing media. Around 18.9% of all protein‐encoding genes were differentially expressed in the experimental conditions. Proline is catabolized by K. phaffii even in the presence of other nitrogen, carbon and energy sources. This results in the repression of genes involved in the utilization of other element sources, namely methanol. We also found that the repression of AOX1 gene promoter with proline can be partially reversed by the deletion of the KpPUT4.2 gene.",
keywords = "Alcohol oxidase, AOX1 promoter, GS115, Komagataella phaffii, Methanol metabolism, Pichia pastoris, Proline, X‐33",
author = "Andrey Rumyantsev and Anton Sidorin and Artemii Volkov and Shanaa, {Ousama Al} and Elena Sambuk and Marina Padkina",
note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = jan,
doi = "10.3390/microorganisms10010067",
language = "English",
volume = "10",
journal = "Microorganisms",
issn = "2076-2607",
publisher = "MDPI AG",
number = "1",

}

RIS

TY - JOUR

T1 - Transcriptome analysis unveils the effects of proline on gene expression in the yeast komagataella phaffii

AU - Rumyantsev, Andrey

AU - Sidorin, Anton

AU - Volkov, Artemii

AU - Shanaa, Ousama Al

AU - Sambuk, Elena

AU - Padkina, Marina

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/1

Y1 - 2022/1

N2 - Komagataella phaffii yeast is one of the most important biocompounds producing micro-organisms in modern biotechnology. Optimization of media recipes and cultivation strategies is key to successful synthesis of recombinant proteins. The complex effects of proline on gene expression in the yeast K. phaffii was analyzed on the transcriptome level in this work. Our analysis revealed drastic changes in gene expression when K. phaffii was grown in proline‐containing media in comparison to ammonium sulphate‐containing media. Around 18.9% of all protein‐encoding genes were differentially expressed in the experimental conditions. Proline is catabolized by K. phaffii even in the presence of other nitrogen, carbon and energy sources. This results in the repression of genes involved in the utilization of other element sources, namely methanol. We also found that the repression of AOX1 gene promoter with proline can be partially reversed by the deletion of the KpPUT4.2 gene.

AB - Komagataella phaffii yeast is one of the most important biocompounds producing micro-organisms in modern biotechnology. Optimization of media recipes and cultivation strategies is key to successful synthesis of recombinant proteins. The complex effects of proline on gene expression in the yeast K. phaffii was analyzed on the transcriptome level in this work. Our analysis revealed drastic changes in gene expression when K. phaffii was grown in proline‐containing media in comparison to ammonium sulphate‐containing media. Around 18.9% of all protein‐encoding genes were differentially expressed in the experimental conditions. Proline is catabolized by K. phaffii even in the presence of other nitrogen, carbon and energy sources. This results in the repression of genes involved in the utilization of other element sources, namely methanol. We also found that the repression of AOX1 gene promoter with proline can be partially reversed by the deletion of the KpPUT4.2 gene.

KW - Alcohol oxidase

KW - AOX1 promoter

KW - GS115

KW - Komagataella phaffii

KW - Methanol metabolism

KW - Pichia pastoris

KW - Proline

KW - X‐33

UR - http://www.scopus.com/inward/record.url?scp=85121816715&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d2a20ef7-d329-3181-9b28-813cec0e3d7c/

U2 - 10.3390/microorganisms10010067

DO - 10.3390/microorganisms10010067

M3 - Article

AN - SCOPUS:85121816715

VL - 10

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 1

M1 - 67

ER -

ID: 91714759