Research output: Contribution to journal › Article › peer-review
Transcriptome analysis of crown gall in radish (Raphanus sativus L.) inbred lines. / Tkachenko, Alexander A.; Gancheva, Maria S.; Tvorogova, Varvara E.; Danilov, Lavrenty G.; Predeus, Alexander V.; Dodueva, Irina E.; Lutova, Ludmila L.
In: Annals of Applied Biology, Vol. 178, No. 3, 05.2021, p. 527-548.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Transcriptome analysis of crown gall in radish (Raphanus sativus L.) inbred lines
AU - Tkachenko, Alexander A.
AU - Gancheva, Maria S.
AU - Tvorogova, Varvara E.
AU - Danilov, Lavrenty G.
AU - Predeus, Alexander V.
AU - Dodueva, Irina E.
AU - Lutova, Ludmila L.
N1 - Publisher Copyright: © 2021 Association of Applied Biologists
PY - 2021/5
Y1 - 2021/5
N2 - Studying aberrations of normal developmental processes like plant tumour initiation provides a great opportunity for understanding of systemic control of plant cells differentiation. Most well-studied example of plant tumour is the crown gall caused by Agrobacterium tumefaciens; much less studied are spontaneous tumours developing in plants with certain genotype, regardless of agrobacterial infection. In the present study, we used RNA-seq to analyse the changes in gene expression at the early stages of development of A. tumefaciens-induced tumours in radish seedlings. In our studies we used two closely related radish inbred lines contrasting in their ability to form spontaneous tumours. RNA-seq and qPCR analysis showed significant changes in the expression levels for numerous genes involved in different metabolic and developmental processes, and Gene Ontology analysis indicated many biological processes probably involved in the crown gall development in radish. For example, a unique result of our research is upregulation of genes related to photomorphogenesis and downregulation of senescence-associated genes in the young crown gall tumours. Differences in gene expression between the analysed radish lines included upregulation of pathways related to chromatin remodelling and protein targeting to chloroplast in the tumorous line along with downregulation of pathways of chaperone-mediated complex assembly and anthocyanin biosynthesis.
AB - Studying aberrations of normal developmental processes like plant tumour initiation provides a great opportunity for understanding of systemic control of plant cells differentiation. Most well-studied example of plant tumour is the crown gall caused by Agrobacterium tumefaciens; much less studied are spontaneous tumours developing in plants with certain genotype, regardless of agrobacterial infection. In the present study, we used RNA-seq to analyse the changes in gene expression at the early stages of development of A. tumefaciens-induced tumours in radish seedlings. In our studies we used two closely related radish inbred lines contrasting in their ability to form spontaneous tumours. RNA-seq and qPCR analysis showed significant changes in the expression levels for numerous genes involved in different metabolic and developmental processes, and Gene Ontology analysis indicated many biological processes probably involved in the crown gall development in radish. For example, a unique result of our research is upregulation of genes related to photomorphogenesis and downregulation of senescence-associated genes in the young crown gall tumours. Differences in gene expression between the analysed radish lines included upregulation of pathways related to chromatin remodelling and protein targeting to chloroplast in the tumorous line along with downregulation of pathways of chaperone-mediated complex assembly and anthocyanin biosynthesis.
KW - Agrobacterium tumefaciens
KW - Raphanus sativus
KW - crown gall
KW - transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85099813473&partnerID=8YFLogxK
U2 - 10.1111/aab.12669
DO - 10.1111/aab.12669
M3 - Article
AN - SCOPUS:85099813473
VL - 178
SP - 527
EP - 548
JO - Annals of Applied Biology
JF - Annals of Applied Biology
SN - 0003-4746
IS - 3
ER -
ID: 71623855