TY - JOUR

T1 - Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus

AU - Antipchik, Mariia

AU - Polyakov, Dmitry

AU - Sinitsyna, Ekaterina

AU - Dzhuzha , Apollinariia

AU - Shavlovsky , Mikhail

AU - Korzhikova-Vlakh , Evgenia

AU - Tennikova, Tatiana

N1 - Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/5

Y1 - 2020/5

N2 - The early diagnostics of hepatitis C virus (HCV) infections is currently one of the most highly demanded medical tasks. This study is devoted to the development of biochips (microarrays) that can be applied for the detection of HCV. The analytical platforms of suggested devices were based on macroporous poly(glycidyl methacrylate-co-di(ethylene glycol) dimethacrylate) monolithic material. The biochips were obtained by the covalent immobilization of specific probes spotted onto the surface of macroporous monolithic platforms. Using the developed biochips, different variants of bioassay were investigated. This study was carried out using hepatitis C virus-mimetic particles (VMPs) representing polymer nanoparticles with a size close to HCV and bearing surface virus antigen (E2 protein). At the first step, the main parameters of bioassay were optimized. Additionally, the dissociation constants were calculated for the pairs “ligand–receptor” and “antigen–antibody” formed at the surface of biochips. As a result of this study, the analysis of VMPs in model buffer solution and human blood plasma was carried out in a format of direct and “sandwich” approaches. It was found that bioassay efficacy appeared to be similar for both the model medium and real biological fluid. Finally, limit of detection (LOD), limit of quantification (LOQ), spot-to-spot and biochip-to-biochip reproducibility for the developed systems were evaluated.

AB - The early diagnostics of hepatitis C virus (HCV) infections is currently one of the most highly demanded medical tasks. This study is devoted to the development of biochips (microarrays) that can be applied for the detection of HCV. The analytical platforms of suggested devices were based on macroporous poly(glycidyl methacrylate-co-di(ethylene glycol) dimethacrylate) monolithic material. The biochips were obtained by the covalent immobilization of specific probes spotted onto the surface of macroporous monolithic platforms. Using the developed biochips, different variants of bioassay were investigated. This study was carried out using hepatitis C virus-mimetic particles (VMPs) representing polymer nanoparticles with a size close to HCV and bearing surface virus antigen (E2 protein). At the first step, the main parameters of bioassay were optimized. Additionally, the dissociation constants were calculated for the pairs “ligand–receptor” and “antigen–antibody” formed at the surface of biochips. As a result of this study, the analysis of VMPs in model buffer solution and human blood plasma was carried out in a format of direct and “sandwich” approaches. It was found that bioassay efficacy appeared to be similar for both the model medium and real biological fluid. Finally, limit of detection (LOD), limit of quantification (LOQ), spot-to-spot and biochip-to-biochip reproducibility for the developed systems were evaluated.

KW - macroporous monolithic polymer layers

KW - Biochips

KW - MICROARRAY

KW - virus-mimetic particles

KW - Hepatitis C

KW - Macroporous monolithic polymer layers

KW - Virus-mimetic particles

KW - Microarray

KW - ANTIBODIES

KW - ARRAY

KW - ASSAY

KW - PARTICLES

KW - hepatitis C

KW - MONOLITHS

KW - IMMOBILIZATION

KW - ACCURACY

KW - biochips

KW - microarray

KW - PURIFICATION

KW - MUTATIONS

KW - PROTEIN MICROARRAY

UR - http://www.scopus.com/inward/record.url?scp=85084412291&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/f3aa6f0a-9b69-320e-a8b7-297567a97b81/

U2 - 10.3390/s20092719

DO - 10.3390/s20092719

M3 - Article

VL - 20

JO - Sensors

JF - Sensors

SN - 1424-3210

IS - 9

M1 - 2719

ER -