TY - JOUR

T1 - Tissue homeostasis in sponges: Quantitative analysis of cell proliferation and apoptosis

AU - Мельников , Николай

AU - Большаков, Федор Васильевич

AU - Фролова, В.С.

AU - Ересковский, Александр Вадимович

AU - Скоренцева, Ксения Витальевна

AU - Саидова, Алина

AU - Лавров, Андрей Игоревич

N1 - Publisher Copyright: © 2022 Wiley Periodicals LLC.

PY - 2022/9

Y1 - 2022/9

N2 - Tissues of multicellular animals are maintained due to a tight balance between cell proliferation and programmed cell death. Sponges are early branching metazoans essential to understanding the key mechanisms of tissue homeostasis. This article is dedicated to the comparative analysis of proliferation and apoptosis in intact tissues of two sponges, Halisarca dujardinii (class Demospongiae) and Leucosolenia variabilis (class Calcarea). Labeled nucleotides EdU and anti-phosphorylated histone 3 antibodies reveal a considerable number of cycling cells in intact tissues of both species. Quantitative DNA staining reveals the classic cell cycle distribution curve. The main type of cycling cells are choanocytes - flagellated cells of the aquiferous system. The rate of proliferation remains constant throughout various areas of sponge bodies that contain choanocytes. The EdU tracking experiments conducted in H. dujardinii indicate that choanocytes may give rise to mesohyl cells through migration. The number of apoptotic cells in tissues of both species is insignificant, although being comparable to the renewing tissues of other animals. In vivo studies with tetramethylrhodamine ethyl ester and CellEvent Caspase-3/7 indicate that apoptosis might be independent of mitochondrial outer membrane permeabilization. Altogether, a combination of confocal laser scanning microscopy and flow cytometry provides a quantitative description of cell proliferation and apoptosis in sponges displaying either rapid growth or cell turnover.

AB - Tissues of multicellular animals are maintained due to a tight balance between cell proliferation and programmed cell death. Sponges are early branching metazoans essential to understanding the key mechanisms of tissue homeostasis. This article is dedicated to the comparative analysis of proliferation and apoptosis in intact tissues of two sponges, Halisarca dujardinii (class Demospongiae) and Leucosolenia variabilis (class Calcarea). Labeled nucleotides EdU and anti-phosphorylated histone 3 antibodies reveal a considerable number of cycling cells in intact tissues of both species. Quantitative DNA staining reveals the classic cell cycle distribution curve. The main type of cycling cells are choanocytes - flagellated cells of the aquiferous system. The rate of proliferation remains constant throughout various areas of sponge bodies that contain choanocytes. The EdU tracking experiments conducted in H. dujardinii indicate that choanocytes may give rise to mesohyl cells through migration. The number of apoptotic cells in tissues of both species is insignificant, although being comparable to the renewing tissues of other animals. In vivo studies with tetramethylrhodamine ethyl ester and CellEvent Caspase-3/7 indicate that apoptosis might be independent of mitochondrial outer membrane permeabilization. Altogether, a combination of confocal laser scanning microscopy and flow cytometry provides a quantitative description of cell proliferation and apoptosis in sponges displaying either rapid growth or cell turnover.

KW - apoptosis

KW - calcarea

KW - cell proliferation

KW - cell turnover

KW - demospongiae

KW - porifera

KW - STEM-CELLS

KW - TURNOVER

KW - DEATH

KW - CONSERVATION

KW - EVOLUTION

KW - MITOCHONDRIAL PATHWAY

KW - KINETICS

KW - DIFFERENTIATION

KW - EXPRESSION

KW - CYCLE

UR - http://www.scopus.com/inward/record.url?scp=85128747389&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/f059d1e5-4057-330c-92d2-b123ccd22f2c/

U2 - 10.1002/jez.b.23138

DO - 10.1002/jez.b.23138

M3 - Article

VL - 338

SP - 360

EP - 381

JO - Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

JF - Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

SN - 1552-5007

IS - 6

ER -