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Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line. / Dosch, John; Hadley, Elise; Wiese, Cal; Soderberg, Marissa; Houwman, Tori; Ding, Kai; Kharazova, Alexandra; Collins, John L.; van Knippenberg, Bart; Gregory, Carl; Kofman, Alexander.

In: Cell Cycle, Vol. 17, No. 2, 17.01.2018, p. 174-181.

Research output: Contribution to journal › Article › peer-review

Harvard

Dosch, J, Hadley, E, Wiese, C, Soderberg, M, Houwman, T, Ding, K, Kharazova, A, Collins, JL, van Knippenberg, B, Gregory, C & Kofman, A 2018, 'Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line', Cell Cycle, vol. 17, no. 2, pp. 174-181. https://doi.org/10.1080/15384101.2017.1395535

APA

Dosch, J., Hadley, E., Wiese, C., Soderberg, M., Houwman, T., Ding, K., Kharazova, A., Collins, J. L., van Knippenberg, B., Gregory, C., & Kofman, A. (2018). Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line. Cell Cycle, 17(2), 174-181. https://doi.org/10.1080/15384101.2017.1395535

Vancouver

Dosch J, Hadley E, Wiese C, Soderberg M, Houwman T, Ding K et al. Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line. Cell Cycle. 2018 Jan 17;17(2):174-181. https://doi.org/10.1080/15384101.2017.1395535

Author

Dosch, John ; Hadley, Elise ; Wiese, Cal ; Soderberg, Marissa ; Houwman, Tori ; Ding, Kai ; Kharazova, Alexandra ; Collins, John L. ; van Knippenberg, Bart ; Gregory, Carl ; Kofman, Alexander. / Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line. In: Cell Cycle. 2018 ; Vol. 17, No. 2. pp. 174-181.

BibTeX

@article{9b3a55e2fd33499488443ce00124e4df,
title = "Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line",
abstract = "Cancer stem cells resemble normal tissue-specific stem cells in many aspects, such as self-renewal and plasticity. Like their non-malignant counterparts, cancer stem cells are suggested to exhibit a relative quiescence. The established cancer cell lines reportedly harbor slow-proliferating cells that are positive for some cancer stem cells markers. However, the fate of these cells and their progeny remains unknown. We used time-lapse microscopy and the contrast-based segmentation algorithm to identify and monitor actively dividing and non-dividing cells in human osteosarcoma MG-63 cell line. Within the monitored field of view the non-dividing cells were represented by three cells that never divided, and one cell that attempted to divide, but failed cytokinesis, and later, after significantly prolonged division, produced the progeny with enlarged segmented nuclei, thus pointing to a possible mitotic catastrophe. Together, these cells initially constituted about 6.2% of the total number of seeded cells, yet only 0.02% of all cells at the end of the observation period when cells became confluent. Non-dividing cells were characterized by rounded shape, dark nuclei, random cytoplasmic streaming and subtle oscillatory movement, however, they did not migrate and rarely formed cell-cell contacts as compared to actively dividing cells. Our data indicate that the observed non-dividing MG-63 cells do not have a growth advantage over other cells and, therefore, they do not contribute to the cancer stem cells pool.",
keywords = "aneuploidy, cancer, cell-cell interaction, Non-dividing, quiescent, stem cells, time-lapse microscopy",
author = "John Dosch and Elise Hadley and Cal Wiese and Marissa Soderberg and Tori Houwman and Kai Ding and Alexandra Kharazova and Collins, {John L.} and {van Knippenberg}, Bart and Carl Gregory and Alexander Kofman",
year = "2018",
month = jan,
day = "17",
doi = "10.1080/15384101.2017.1395535",
language = "English",
volume = "17",
pages = "174--181",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Landes Bioscience",
number = "2",

}

RIS

TY - JOUR

T1 - Time-lapse microscopic observation of non-dividing cells in cultured human osteosarcoma MG-63 cell line

AU - Dosch, John

AU - Hadley, Elise

AU - Wiese, Cal

AU - Soderberg, Marissa

AU - Houwman, Tori

AU - Ding, Kai

AU - Kharazova, Alexandra

AU - Collins, John L.

AU - van Knippenberg, Bart

AU - Gregory, Carl

AU - Kofman, Alexander

PY - 2018/1/17

Y1 - 2018/1/17

N2 - Cancer stem cells resemble normal tissue-specific stem cells in many aspects, such as self-renewal and plasticity. Like their non-malignant counterparts, cancer stem cells are suggested to exhibit a relative quiescence. The established cancer cell lines reportedly harbor slow-proliferating cells that are positive for some cancer stem cells markers. However, the fate of these cells and their progeny remains unknown. We used time-lapse microscopy and the contrast-based segmentation algorithm to identify and monitor actively dividing and non-dividing cells in human osteosarcoma MG-63 cell line. Within the monitored field of view the non-dividing cells were represented by three cells that never divided, and one cell that attempted to divide, but failed cytokinesis, and later, after significantly prolonged division, produced the progeny with enlarged segmented nuclei, thus pointing to a possible mitotic catastrophe. Together, these cells initially constituted about 6.2% of the total number of seeded cells, yet only 0.02% of all cells at the end of the observation period when cells became confluent. Non-dividing cells were characterized by rounded shape, dark nuclei, random cytoplasmic streaming and subtle oscillatory movement, however, they did not migrate and rarely formed cell-cell contacts as compared to actively dividing cells. Our data indicate that the observed non-dividing MG-63 cells do not have a growth advantage over other cells and, therefore, they do not contribute to the cancer stem cells pool.

AB - Cancer stem cells resemble normal tissue-specific stem cells in many aspects, such as self-renewal and plasticity. Like their non-malignant counterparts, cancer stem cells are suggested to exhibit a relative quiescence. The established cancer cell lines reportedly harbor slow-proliferating cells that are positive for some cancer stem cells markers. However, the fate of these cells and their progeny remains unknown. We used time-lapse microscopy and the contrast-based segmentation algorithm to identify and monitor actively dividing and non-dividing cells in human osteosarcoma MG-63 cell line. Within the monitored field of view the non-dividing cells were represented by three cells that never divided, and one cell that attempted to divide, but failed cytokinesis, and later, after significantly prolonged division, produced the progeny with enlarged segmented nuclei, thus pointing to a possible mitotic catastrophe. Together, these cells initially constituted about 6.2% of the total number of seeded cells, yet only 0.02% of all cells at the end of the observation period when cells became confluent. Non-dividing cells were characterized by rounded shape, dark nuclei, random cytoplasmic streaming and subtle oscillatory movement, however, they did not migrate and rarely formed cell-cell contacts as compared to actively dividing cells. Our data indicate that the observed non-dividing MG-63 cells do not have a growth advantage over other cells and, therefore, they do not contribute to the cancer stem cells pool.

KW - aneuploidy

KW - cancer

KW - cell-cell interaction

KW - Non-dividing

KW - quiescent

KW - stem cells

KW - time-lapse microscopy

UR - http://www.scopus.com/inward/record.url?scp=85039066045&partnerID=8YFLogxK

U2 - 10.1080/15384101.2017.1395535

DO - 10.1080/15384101.2017.1395535

M3 - Article

C2 - 29169283

AN - SCOPUS:85039066045

VL - 17

SP - 174

EP - 181

JO - Cell Cycle

JF - Cell Cycle

SN - 1538-4101

IS - 2

ER -

ID: 41025435