Research output: Contribution to journal › Article › peer-review
Thermosensitive phosphorus(V) porphyrin: Toward subcellular ratiometric optical temperature sensing. / Колесников, Илья Евгеньевич; Калиничев, Алексей Андреевич; Соломатина, Анастасия Игоревна; Курочкин, Михаил Алексеевич; Мешков, И.Н.; Колесников, Евгений Ю.; Gorbunova, Yulia G.
In: Sensors and Actuators, A: Physical, Vol. 347, 113917, 01.11.2022.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Thermosensitive phosphorus(V) porphyrin: Toward subcellular ratiometric optical temperature sensing
AU - Колесников, Илья Евгеньевич
AU - Калиничев, Алексей Андреевич
AU - Соломатина, Анастасия Игоревна
AU - Курочкин, Михаил Алексеевич
AU - Мешков, И.Н.
AU - Колесников, Евгений Ю.
AU - Gorbunova, Yulia G.
N1 - Publisher Copyright: © 2022 Elsevier B.V.
PY - 2022/11/1
Y1 - 2022/11/1
N2 - Luminescence thermometry has attracted a lot of attention due to its ability to provide temperature sensing with high spatial, temporal, and thermal resolution. This technique is the most promising for probing changes in local temperature in living cells to monitor thermogenesis and to study cellular activity. Despite rapid progress in this field, there is still a lack of optical thermometers capable of thermal sensing at the subcellular level, especially owning additional functionality. Here, cationic (meso-mono(4-pyridyl)-triphenylporphyrinato)phosphorus(V) bromide MPyPP(OH) 2 was used as subcellular ratiometric thermal sensor in CHO-K1 and HeLa cells within the biological temperature range (30–44 oC). Introduction of porphyrin into cells resulted in its dephosphorylation and change of luminescence properties. Ratiometric sensing was performed using peak-to-peak and peak-to-valley ratios. Thermometric performance was evaluated in terms of absolute and relative sensitivities, temperature resolution. Relative thermal sensitivity and temperature resolution reached 0.61% K -1 and 0.1 oC, respectively in the case of CHO-K1 cells.
AB - Luminescence thermometry has attracted a lot of attention due to its ability to provide temperature sensing with high spatial, temporal, and thermal resolution. This technique is the most promising for probing changes in local temperature in living cells to monitor thermogenesis and to study cellular activity. Despite rapid progress in this field, there is still a lack of optical thermometers capable of thermal sensing at the subcellular level, especially owning additional functionality. Here, cationic (meso-mono(4-pyridyl)-triphenylporphyrinato)phosphorus(V) bromide MPyPP(OH) 2 was used as subcellular ratiometric thermal sensor in CHO-K1 and HeLa cells within the biological temperature range (30–44 oC). Introduction of porphyrin into cells resulted in its dephosphorylation and change of luminescence properties. Ratiometric sensing was performed using peak-to-peak and peak-to-valley ratios. Thermometric performance was evaluated in terms of absolute and relative sensitivities, temperature resolution. Relative thermal sensitivity and temperature resolution reached 0.61% K -1 and 0.1 oC, respectively in the case of CHO-K1 cells.
KW - Dephosphorylation
KW - Fluorescent thermometry
KW - Subcellular sensing
KW - Thermal sensitivity
KW - Water-soluble phosphorus(V) porphyrin
UR - http://www.scopus.com/inward/record.url?scp=85139593904&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/9fc13b07-c0d0-302f-9ff4-2c03d48879a8/
U2 - 10.1016/j.sna.2022.113917
DO - 10.1016/j.sna.2022.113917
M3 - Article
VL - 347
JO - Sensors and Actuators, A: Physical
JF - Sensors and Actuators, A: Physical
SN - 0924-4247
M1 - 113917
ER -
ID: 99569870